| Literature DB >> 27438134 |
Hiroyasu Kitashiba1, Kumiko Taguchi2, Ikuyo Kaneko2, Kiyofumi Inaba2, Shuji Yokoi3,4, Yoshihito Takahata3, Takeshi Nishio2.
Abstract
KEY MESSAGE: We identified three physical positions associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis. We also confirmed their genetic effects on the embryo yield. Isolated microspore culture is well utilized for the production of haploid or doubled-haploid plants in Brassica crops. Brassica rapa cv. 'Ho Mei' is one of the most excellent cultivars in embryo yield of microspore culture. To identify the loci associated with microspore embryogenesis, segregation analysis of 154 DNA markers anchored to B. rapa chromosomes (A01-A10) was performed using a population of microspore-derived embryos obtained from an F1 hybrid between 'CR-Seiga', a low yield cultivar in microspore-derived embryos, and 'Ho Mei'. Three regions showing significant segregation distortion with increasing 'Ho Mei' alleles were detected on A05, A08 and A09, although these regions showed the expected Mendelian segregation ratio in an F2 population. The additive effect of alleles in these regions on embryo yield was confirmed in a BC3F1 population. One region on A08 containing Br071-5c had a higher effect than the other regions. Polymorphism of nucleotide sequences around the Br071-5c locus was investigated to find the gene possibly responsible for efficient embryogenesis from microspores.Entities:
Keywords: Brassica rapa; Microspore culture; Microspore-derived embryo; Segregation distortion
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Year: 2016 PMID： 27438134 DOI： 10.1007/s00299-016-2029-4
Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570