Alice Buss Cruz1, Heloísa da Silva Pitz2, Bruno Veber1, Larissa Alida Bini1, Marcelo Maraschin2, Ana Lúcia Bertarello Zeni1. 1. a Laboratório de Análise de Substâncias Bioativas, Departamento de Ciências Naturais , Universidade Regional de Blumenau , Blumenau , SC , Brazil. 2. b Laboratório de Morfogênese e Bioquímica Vegetal, Centro de Ciências Agrárias , Universidade Federal de Santa Catarina , Itacorubi, Florianópolis , SC , Brazil.
Abstract
CONTEXT: Cardiovascular disease is the leading cause of death worldwide and the consumption of red cabbage (Brassica oleracea var. capitata f. rubra DC. - Brassicaceae) has been linked with the reduction risk of chronic diseases. OBJECTIVE: The present study assesses the bioactive metabolites and hypolipidemic effect of red cabbage on rats. MATERIALS AND METHODS: The content of total phenols, flavonoids, anthocyanins, carotenoids, ascorbic acid and antioxidant capacity were assessed, while individual phenolic acids and flavonoids were detected using reverse phase-high performance liquid chromatography (HPLC) analysis. Acute hypolipidemic activity of aqueous extract of red cabbage (RC - 125, 250 and 500 mg/kg) was investigated using a Triton WR-1339 (400 mg/kg) induced hyperlipidemic Wistar rats compared to fenofibrate (65 mg/kg). RESULTS: The HPLC analysis of extracts revealed eight phenolic acids, gallic, protocatechuic, p-hydroxybenzoic, m-coumaric, syringic, caffeic, cinnamic, dicaffeoylquinic and three flavonoids, epicatechin, epigallocatechin, gallocatechin. Furthermore, the aqueous extract showed higher amounts of total phenolics (116.00 mg/g), flavonoids (161.32 μg/g) and, antioxidant activity (87.19%) than the hydromethanolic (89.33 mg/g, 123.34 μg/g and 75.07%), respectively. The RC significantly (p < 0.001) ameliorated the levels of cholesterol, triglycerides and lipoproteins alterations in hyperlipidemic rats without toxicity. DISCUSSION AND CONCLUSION: Herein, the RC presented the higher amounts of phenolics and flavonoids comparing with the hydromethanolic extract. Additionally, the RC showed as the majority compounds, dicaffeoylquinic and cinnamic acids, and the flavonoids epicatechin and gallocatechin. Furthermore, the RC demonstrated a beneficial effect against hypercholesterolemia and hypertriglyceridemia, demonstrating its potential therapeutic effect on these risk factors of cardiovascular diseases.
CONTEXT: Cardiovascular disease is the leading cause of death worldwide and the consumption of red cabbage (Brassica oleracea var. capitataf. rubra DC. - Brassicaceae) has been linked with the reduction risk of chronic diseases. OBJECTIVE: The present study assesses the bioactive metabolites and hypolipidemic effect of red cabbage on rats. MATERIALS AND METHODS: The content of total phenols, flavonoids, anthocyanins, carotenoids, ascorbic acid and antioxidant capacity were assessed, while individual phenolic acids and flavonoids were detected using reverse phase-high performance liquid chromatography (HPLC) analysis. Acute hypolipidemic activity of aqueous extract of red cabbage (RC - 125, 250 and 500 mg/kg) was investigated using a Triton WR-1339 (400 mg/kg) induced hyperlipidemic Wistar rats compared to fenofibrate (65 mg/kg). RESULTS: The HPLC analysis of extracts revealed eight phenolic acids, gallic, protocatechuic, p-hydroxybenzoic, m-coumaric, syringic, caffeic, cinnamic, dicaffeoylquinic and three flavonoids, epicatechin, epigallocatechin, gallocatechin. Furthermore, the aqueous extract showed higher amounts of total phenolics (116.00 mg/g), flavonoids (161.32 μg/g) and, antioxidant activity (87.19%) than the hydromethanolic (89.33 mg/g, 123.34 μg/g and 75.07%), respectively. The RC significantly (p < 0.001) ameliorated the levels of cholesterol, triglycerides and lipoproteins alterations in hyperlipidemic rats without toxicity. DISCUSSION AND CONCLUSION: Herein, the RC presented the higher amounts of phenolics and flavonoids comparing with the hydromethanolic extract. Additionally, the RC showed as the majority compounds, dicaffeoylquinic and cinnamic acids, and the flavonoidsepicatechin and gallocatechin. Furthermore, the RC demonstrated a beneficial effect against hypercholesterolemia and hypertriglyceridemia, demonstrating its potential therapeutic effect on these risk factors of cardiovascular diseases.
Authors: Michał Majewski; Bernadetta Lis; Jerzy Juśkiewicz; Katarzyna Ognik; Małgorzata Borkowska-Sztachańska; Dariusz Jedrejek; Anna Stochmal; Beata Olas Journal: Antioxidants (Basel) Date: 2020-02-03
Authors: Waseem Khalid; Muhammad Sajid Arshad; Muhammad Modassar Ali Nawaz Ranjha; Maria Barbara Różańska; Shafeeqa Irfan; Bakhtawar Shafique; Muhammad Abdul Rahim; Muhammad Zubair Khalid; Gholamreza Abdi; Przemysław Łukasz Kowalczewski Journal: Open Life Sci Date: 2022-09-08 Impact factor: 1.311