Isaac Farias Cansanção1,2, Ana Paula Santos do Carmo3,4, Robério Dias Leite5,6, Rosana Deyse Ponte Portela3,4, Sérgio de Sá Leitão Paiva Júnior7, Valdir de Queiroz Balbino7, Silvia Helena Barem Rabenhorst3,4. 1. Medicine Collegiate, Federal University of San Francisco Valley, Paulo Afonso, Bahia, Brazil. isaac.farias@univasf.edu.br. 2. Centro de Treinamento Profissional de Paulo Afonso (CFPPA), Rua Aurora, Bloco 27, Lote 3, Bairro Alves de Souza, Paulo Afonso, Brazil. isaac.farias@univasf.edu.br. 3. Molecular Genetics Laboratory, Department of Pathology and Forensic Medicine, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil. 4. , Rua Coronel Nunes de Melo, 1315, Bairro Rodolfo Teófilo, Fortaleza, Brazil. 5. São José Hospital of Infectious Diseases, Fortaleza, Ceará, Brazil. 6. , Rua Nestor Barbosa, 315, Bairro Parquelândia, Fortaleza, Brazil. 7. Genetics Department, Laboratory of Bioinformatics and Evolutionary Biology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Abstract
OBJECTIVE: In this study, single nucleotide polymorphisms (SNP) of interleukin (IL) 1β -511C>T, IL1RN VNTR 86 bp, IL6 -174G>C, IL10 -819C>T and TNFα -308G>A were analyzed by PCR-RFLP with symptoms of dengue with the clinical features. SUBJECTS: 196 individuals admitted to the São José Infectious Diseases Hospital with suspected dengue infection. Dengue was confirmed in 111 of the patients. The control group consisted of 85 other individuals confirmed without dengue. RESULTS: It was demonstrated that the presence the T allele of IL1β (P < 0.05) was associated with susceptibility to developing the disease. Other results also suggested that the polymorphism in the combinations IL6 × IL1β (C and T alleles, respectively), IL1β (T allele) × IL1RN (*2/*2 genotype), IL6 (C allele) × TNFα (A allele), IL10 (C/T genotype) × TNFα (A/A genotype) (P < 0.01, P = 0.01, P < 0.05 and P = 0.03, respectively) were associated with predisposition to developing the disease and its symptoms. CONCLUSIONS: In summary, the findings of this study in a Brazilian population point out the importance of studies of combinations of polymorphisms in the development of dengue, which can increase the risk of dengue infection and its severity.
OBJECTIVE: In this study, single nucleotide polymorphisms (SNP) of interleukin (IL) 1β -511C>T, IL1RN VNTR 86 bp, IL6 -174G>C, IL10 -819C>T and TNFα -308G>A were analyzed by PCR-RFLP with symptoms of dengue with the clinical features. SUBJECTS: 196 individuals admitted to the São José Infectious Diseases Hospital with suspected dengue infection. Dengue was confirmed in 111 of the patients. The control group consisted of 85 other individuals confirmed without dengue. RESULTS: It was demonstrated that the presence the T allele of IL1β (P < 0.05) was associated with susceptibility to developing the disease. Other results also suggested that the polymorphism in the combinations IL6 × IL1β (C and T alleles, respectively), IL1β (T allele) × IL1RN (*2/*2 genotype), IL6 (C allele) × TNFα (A allele), IL10 (C/T genotype) × TNFα (A/A genotype) (P < 0.01, P = 0.01, P < 0.05 and P = 0.03, respectively) were associated with predisposition to developing the disease and its symptoms. CONCLUSIONS: In summary, the findings of this study in a Brazilian population point out the importance of studies of combinations of polymorphisms in the development of dengue, which can increase the risk of dengue infection and its severity.
Authors: Ana B Perez; Beatriz Sierra; Gissel Garcia; Eglis Aguirre; Nina Babel; Mayling Alvarez; Licel Sanchez; Luis Valdes; Hans D Volk; Maria G Guzman Journal: Hum Immunol Date: 2010-08-21 Impact factor: 2.850
Authors: H J M Kerkhof; M Doherty; N K Arden; S B Abramson; M Attur; S D Bos; C Cooper; E M Dennison; S A Doherty; E Evangelou; D J Hart; A Hofman; K Javaid; I Kerna; K Kisand; M Kloppenburg; S Krasnokutsky; R A Maciewicz; I Meulenbelt; K R Muir; F Rivadeneira; J Samuels; M Sezgin; E Slagboom; A J P Smith; T D Spector; A Tamm; A Tamm; A G Uitterlinden; M Wheeler; G Zhai; W Zhang; J B J van Meurs; A M Valdes Journal: Osteoarthritis Cartilage Date: 2010-12-10 Impact factor: 6.576
Authors: P F Vasconcelos; E S Travassos da Rosa; J F Travassos da Rosa; R B de Freitas; N Dégallier; S G Rodrigues; A P Travassos da Rosa Journal: Rev Inst Med Trop Sao Paulo Date: 1993 Mar-Apr Impact factor: 1.846