Literature DB >> 27436111

Improved Proteome and Phosphoproteome Analysis on a Cation Exchanger by a Combined Acid and Salt Gradient.

Jun Adachi1, Kazunari Hashiguchi1, Maiko Nagano1, Misako Sato1, Ayako Sato1, Kazuna Fukamizu1, Yasushi Ishihama2, Takeshi Tomonaga1.   

Abstract

Currently used elution methods for strong cation exchange (SCX) chromatography are based on two principles: salt and pH gradient. In this paper, we report the first observation of peptide elution by acid gradient. The degree of peptide separation using C18-SCX StageTip was greatly improved by our acid and salt-based elution method compared with a salt-based elution method. This development enabled us to identify over 22 000 phosphopeptides from 2 mg of protein without labor-intensive sample preparation. Our method is simple, robust, scalable, and low-cost and can be easily implemented without any special equipment or techniques.

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Year:  2016        PMID： 27436111     DOI： 10.1021/acs.analchem.6b01232

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  22 in total

1.  Magnetite nanoparticles coated with chitosan and polyethylenimine as anion exchanger for sorptive enrichment of phosphopeptides.

Authors:  Dilshad Hussain; Syed Ghulam Musharraf; Batool Fatima; Adeela Saeed; Fahmida Jabeen; Muhammad Naeem Ashiq; Muhammad Najam-Ul-Haq
Journal:  Mikrochim Acta       Date:  2019-11-28       Impact factor: 5.833

Review 2.  Recent advances in phosphoproteomics and application to neurological diseases.

Authors:  Justine V Arrington; Chuan-Chih Hsu; Sarah G Elder; W Andy Tao
Journal:  Analyst       Date:  2017-11-20       Impact factor: 4.616

3.  Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA Coprotease.

Authors:  Matthew F Wipperman; Brook E Heaton; Astha Nautiyal; Oyindamola Adefisayo; Henry Evans; Richa Gupta; Dave van Ditmarsch; Rajesh Soni; Ron Hendrickson; Jeff Johnson; Nevan Krogan; Michael S Glickman
Journal:  Mol Cell       Date:  2018-08-30       Impact factor: 17.970

4.  The FAT10 post-translational modification is involved in the lytic replication of Kaposi's sarcoma-associated herpesvirus.

Authors:  Atsuko Sugimoto; Yuichi Abe; Tadashi Watanabe; Kohei Hosokawa; Jun Adachi; Takeshi Tomonaga; Yasumasa Iwatani; Takayuki Murata; Masahiro Fujimuro
Journal:  J Virol       Date:  2021-02-24       Impact factor: 5.103

5.  S-Like-Phase Cyclin-Dependent Kinases Stabilize the Epstein-Barr Virus BDLF4 Protein To Temporally Control Late Gene Transcription.

Authors:  Yoshitaka Sato; Takahiro Watanabe; Chihiro Suzuki; Yuichi Abe; H M Abdullah Al Masud; Tomoki Inagaki; Masahiro Yoshida; Takeshi Suzuki; Fumi Goshima; Jun Adachi; Takeshi Tomonaga; Takayuki Murata; Hiroshi Kimura
Journal:  J Virol       Date:  2019-04-03       Impact factor: 5.103

6.  Proteomic analysis of AQP11-null kidney: Proximal tubular type polycystic kidney disease.

Authors:  Tatsuya Saito; Yasuko Tanaka; Yoshiyuki Morishita; Kenichi Ishibashi
Journal:  Biochem Biophys Rep       Date:  2017-11-23

7.  TKI-addicted ROS1-rearranged cells are destined to survival or death by the intensity of ROS1 kinase activity.

Authors:  Hayato Ogura; Yuka Nagatake-Kobayashi; Jun Adachi; Takeshi Tomonaga; Naoya Fujita; Ryohei Katayama
Journal:  Sci Rep       Date:  2017-07-17       Impact factor: 4.379

8.  Deep Phospho- and Phosphotyrosine Proteomics Identified Active Kinases and Phosphorylation Networks in Colorectal Cancer Cell Lines Resistant to Cetuximab.

Authors:  Yuichi Abe; Maiko Nagano; Takahisa Kuga; Asa Tada; Junko Isoyama; Jun Adachi; Takeshi Tomonaga
Journal:  Sci Rep       Date:  2017-09-05       Impact factor: 4.379

9.  Improved phosphoproteomic analysis for phosphosignaling and active-kinome profiling in Matrigel-embedded spheroids and patient-derived organoids.

Authors:  Yuichi Abe; Asa Tada; Junko Isoyama; Satoshi Nagayama; Ryoji Yao; Jun Adachi; Takeshi Tomonaga
Journal:  Sci Rep       Date:  2018-07-30       Impact factor: 4.379

10.  Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.

Authors:  Takashi Shiromizu; Hideaki Kume; Mimiko Ishida; Jun Adachi; Masayuki Kano; Hisahiro Matsubara; Takeshi Tomonaga
Journal:  Sci Rep       Date:  2017-10-06       Impact factor: 4.379
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