Literature DB >> 27431247

Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay.

Ying Zheng1, Jen-Chih Hsieh1, Julia Escandon2, George Cotsarelis3.   

Abstract

The hair follicle (HF) is a dynamic structure readily accessible within the skin, and contains various pools of stem cells that have a broad regenerative potential during normal homeostasis and in response to injury. Recent discoveries demonstrating the multipotent capabilities of hair follicle stem cells and the easy access to skin tissue make the HF an attractive source for isolating stem cells and their subsequent application in tissue engineering and regenerative medicine. Here, we describe the isolation and purification of hair follicle bulge stem cells from mouse skin, and hair reconstitution assays that allows the functional analysis of multipotent stem cells.

Entities:  

Keywords:  Bulge; Hair follicle stem cell; Hair reconstitution assay; Regeneration

Mesh：

Substances：

Year:  2016        PMID： 27431247      PMCID： PMC7450485          DOI： 10.1007/978-1-4939-3786-8_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

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Authors:  Ying Zheng; Xiabing Du; Wei Wang; Marylene Boucher; Satish Parimoo; Kurts Stenn
Journal:  J Invest Dermatol       Date:  2005-05       Impact factor: 8.551

2.  Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis.

Authors:  G Cotsarelis; T T Sun; R M Lavker
Journal:  Cell       Date:  1990-06-29       Impact factor: 41.582

3.  Subpopulations of primary adult murine epidermal basal cells sedimented on density gradients.

Authors:  R J Morris; S M Fischer; A J Klein-Szanto; T J Slaga
Journal:  Cell Tissue Kinet       Date:  1990-11

4.  Comparisons of mouse models for hair follicle reconstitution.

Authors:  Yanhua Liang; Kathleen A Silva; Victoria Kennedy; John P Sundberg
Journal:  Exp Dermatol       Date:  2011-10-13       Impact factor: 3.960

5.  Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.

Authors:  Carol S Trempus; Rebecca J Morris; Carl D Bortner; George Cotsarelis; Randall S Faircloth; Jeffrey M Reece; Raymond W Tennant
Journal:  J Invest Dermatol       Date:  2003-04       Impact factor: 8.551

6.  Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice.

Authors:  Ulrike Lichti; Joanna Anders; Stuart H Yuspa
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

7.  In vivo regulation of murine hair growth: insights from grafting defined cell populations onto nude mice.

Authors:  U Lichti; W C Weinberg; L Goodman; S Ledbetter; T Dooley; D Morgan; S H Yuspa
Journal:  J Invest Dermatol       Date:  1993-07       Impact factor: 8.551

8.  Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.

Authors:  Yaping Liu; Stephen Lyle; Zaixin Yang; George Cotsarelis
Journal:  J Invest Dermatol       Date:  2003-11       Impact factor: 8.551

9.  Capturing and profiling adult hair follicle stem cells.

Authors:  Rebecca J Morris; Yaping Liu; Lee Marles; Zaixin Yang; Carol Trempus; Shulan Li; Jamie S Lin; Janet A Sawicki; George Cotsarelis
Journal:  Nat Biotechnol       Date:  2004-03-14       Impact factor: 54.908
  9 in total
  2 in total

1.  Design, synthesis, and biological activity of TLR7-based compounds for chemotherapy-induced alopecia.

Authors:  Jincheng Yang; Kun Chen; Bin Wang; Liudi Wang; Shuya Qi; Weihua Wang
Journal:  Invest New Drugs       Date:  2019-07-04       Impact factor: 3.850

2.  An Ovol2-Zeb1 transcriptional circuit regulates epithelial directional migration and proliferation.

Authors:  Daniel Haensel; Peng Sun; Adam L MacLean; Xianghui Ma; Yuan Zhou; Marc P Stemmler; Simone Brabletz; Geert Berx; Maksim V Plikus; Qing Nie; Thomas Brabletz; Xing Dai
Journal:  EMBO Rep       Date:  2018-11-09       Impact factor: 8.807
  2 in total

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