Damien Bouvier1,2, Thomas Duret1, Mathieu Abbot3,4, Thibault Stiernon3, Bruno Pereira5, Aurélien Coste6, Jean Chazal6, Vincent Sapin7,8,9,10. 1. Department of Biochemistry and Molecular Biology, CHU Clermont-Ferrand, Clermont-Ferrand, France. 2. EA7281-Retinoids, Reproduction Developmental Diseases, School of Medicine, Clermont Université, Université d'Auvergne, Clermont-Ferrand, France. 3. Medical Department, Sports Association Montferrandaise Clermont Auvergne (ASMCA), Clermont-Ferrand, France. 4. Department of Sport Medicine and Functional Explorations, CHU Clermont-Ferrand, Clermont-Ferrand, France. 5. Department of Biostatistics Unit (DRCI), CHU Clermont-Ferrand, Clermont-Ferrand, France. 6. Department of Neurosurgery, CHU Clermont-Ferrand, Clermont-Ferrand, France. 7. Department of Biochemistry and Molecular Biology, CHU Clermont-Ferrand, Clermont-Ferrand, France. vincent.sapin@udamail.fr. 8. EA7281-Retinoids, Reproduction Developmental Diseases, School of Medicine, Clermont Université, Université d'Auvergne, Clermont-Ferrand, France. vincent.sapin@udamail.fr. 9. Laboratoire de Biochimie Médicale, 4R3, Faculté de Médecine, Université d'Auvergne, 28 place Henri-Dunant, BP38, 63001, Clermont Ferrand Cedex, France. vincent.sapin@udamail.fr. 10. Observatoire Médical, FFR-LNR, Marcoussis, France. vincent.sapin@udamail.fr.
Abstract
BACKGROUND: The incidence of concussion in professional direct-contact sports, particularly in rugby, has increased in recent years. To date, cognitive assessment is the most common means of determining whether a concussed player can return to the game. Serum S100B assay, an objective blood test known to be useful in brain injury management, may offer a novel additional approach to the management of concussed male rugby players. OBJECTIVE: The aim of this study was to investigate the S100B utility for the determination of concussion in a professional 15-players-a-side rugby team. METHODS: Thirty-nine male rugby players were included in a prospective study during the 2014-2015 French championship season. Serum sampling was carried out several times at baseline and after a match and/or a concussion, at set times (2, 36 h). Serum S100B concentrations were determined using chemiluminescence immunoassay on a Roche Diagnostics® instrument. RESULTS: The players' basal serum S100B was stable during the season and was not correlated with anthropometric data, body composition, or creatine kinase concentration. A significant increase in S100B concentration within 2 h after a game (without concussion) was observed. This increase was correlated with the number of body collisions during a match. Seventy-seven assays were performed 36 h after a game, including the follow-up of five concussed players. Thirty-six hours after a match, an increase of a minimum of 20 % compared with individual basal concentrations identified concussion with 100 % sensitivity and 81 % specificity. CONCLUSION: S100B measured 36 h after a match is thus a discriminating test to identify concussion in a male rugby player, with a 100 % negative predictive value.
BACKGROUND: The incidence of concussion in professional direct-contact sports, particularly in rugby, has increased in recent years. To date, cognitive assessment is the most common means of determining whether a concussed player can return to the game. Serum S100B assay, an objective blood test known to be useful in brain injury management, may offer a novel additional approach to the management of concussed male rugby players. OBJECTIVE: The aim of this study was to investigate the S100B utility for the determination of concussion in a professional 15-players-a-side rugby team. METHODS: Thirty-nine male rugby players were included in a prospective study during the 2014-2015 French championship season. Serum sampling was carried out several times at baseline and after a match and/or a concussion, at set times (2, 36 h). Serum S100B concentrations were determined using chemiluminescence immunoassay on a Roche Diagnostics® instrument. RESULTS: The players' basal serum S100B was stable during the season and was not correlated with anthropometric data, body composition, or creatine kinase concentration. A significant increase in S100B concentration within 2 h after a game (without concussion) was observed. This increase was correlated with the number of body collisions during a match. Seventy-seven assays were performed 36 h after a game, including the follow-up of five concussed players. Thirty-six hours after a match, an increase of a minimum of 20 % compared with individual basal concentrations identified concussion with 100 % sensitivity and 81 % specificity. CONCLUSION:S100B measured 36 h after a match is thus a discriminating test to identify concussion in a male rugby player, with a 100 % negative predictive value.
Authors: Timothy B Meier; Lindsay D Nelson; Daniel L Huber; Jeffrey J Bazarian; Ronald L Hayes; Michael A McCrea Journal: J Neurotrauma Date: 2017-08-04 Impact factor: 5.269
Authors: Jamie Kearns; Aisling M Ross; Darragh R Walsh; Rachel M Cahalane; Rita Hinchion; Maria C Ryan; Elaine Conway; Tom M Comyns; Ian C Kenny; Eibhlís M O'Connor; Kieran D McGourty; John Joseph Eugene Mulvihill Journal: BMJ Open Sport Exerc Med Date: 2020-11-26
Authors: Timothy B Meier; Daniel L Huber; Luisa Bohorquez-Montoya; Morgan E Nitta; Jonathan Savitz; T Kent Teague; Jeffrey J Bazarian; Ronald L Hayes; Lindsay D Nelson; Michael A McCrea Journal: Ann Neurol Date: 2020-04-20 Impact factor: 10.422