Vishal Sahu1, Abhishek Gupta1, Rahul Kumar1, Tarang Gupta2, Anant Mohan2, Sharmistha Dey3. 1. Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. 2. Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. 3. Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. Electronic address: sharmistha_d@hotmail.com.
Abstract
BACKGROUND: Rac proteins play a major role in tumorogenesis. We quantified Rac1 and Rac1b in serum of non small cell lung cancer (NSCLC) patients. METHODS: The blood of 77 NSCLC patients and 52 healthy controls were collected and quantified the concentration of Rac1 and Rac1b mainly by surface plasmon resonance and it was verify by Western blot analysis. RESULTS: Rac1 and Rac1b were found to be significantly over expressed in serum of NSCLC patients compare to healthy controls. The level of Rac proteins were found to be increased in all stages of cancer. Despite the low survival rate, we managed to collect serum sample of the 18 follow up patients after the therapy, where 11 patients' of CR+PR group showed down regulation of the Rac protein after chemotherapy and unfortunately 80% patients died during the study period. CONCLUSION: The high specificity and sensitivity obtained from ROC analysis for Rac1 and Rac1b envisaged it to be used as a serum diagnostic marker in the early stage of cancer.
BACKGROUND:Rac proteins play a major role in tumorogenesis. We quantified Rac1 and Rac1b in serum of non small cell lung cancer (NSCLC) patients. METHODS: The blood of 77 NSCLCpatients and 52 healthy controls were collected and quantified the concentration of Rac1 and Rac1b mainly by surface plasmon resonance and it was verify by Western blot analysis. RESULTS:Rac1 and Rac1b were found to be significantly over expressed in serum of NSCLCpatients compare to healthy controls. The level of Rac proteins were found to be increased in all stages of cancer. Despite the low survival rate, we managed to collect serum sample of the 18 follow up patients after the therapy, where 11 patients' of CR+PR group showed down regulation of the Rac protein after chemotherapy and unfortunately 80% patients died during the study period. CONCLUSION: The high specificity and sensitivity obtained from ROC analysis for Rac1 and Rac1b envisaged it to be used as a serum diagnostic marker in the early stage of cancer.