Dejun Xu1, Rutao Xu2, Liu He3, Tao Xu4, Zhenyu Zhang5, Dongmei Han1, Jianshi Du6. 1. Department of Vascular and Lymphatic Surgery, Vascular and Lymphatic Surgery, China Japan Union Hospital of Jilin University, Changchun, Jilin, PR China. 2. Department of Vascular Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan, PR China. 3. Department of Anesthesiology, China Japan Union Hospital of Jilin University, Changchun, Jilin, PR China. 4. Department of Gastroenterology, The Fourth Clinical Hospital of Jilin University, Changchun, Jilin, PR China. 5. The Second Department of General Surgery, Armed Police Corps Hospital in Jilin Province, Changchun, Jilin, PR China. 6. Department of Vascular and Lymphatic Surgery, Vascular and Lymphatic Surgery, China Japan Union Hospital of Jilin University, Changchun, Jilin, PR China. Electronic address: dujianshi3043@163.com.
Abstract
BACKGROUND: Unprovoked venous thromboembolism (VTE), generally divided into single and recurrent categories, is a common leading cause of morbidity and mortality in a real-world population. This study was aimed to explore the similarities and differences in the mechanisms of single and recurrent VTE. METHODS: Gene expression data (GSE19151) generated from 63 healthy controls, 32 single, and 38 recurrent VTE patients were analyzed. Differentially expressed genes (DEGs) were screened by Affy package and Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analysis were performed using database for annotation, visualization, and integrated discovery. Based on the Search Tool for the Retrieval of Interacting Genes/Proteins database, protein-protein interaction network was visualized by Cytoscape, and modules were identified by CFinder. Finally, transcription factor regulatory networks were constructed. RESULTS: Totally, 559 and 294 DEGs were obtained from recurrent and single VTE, respectively. There were 202 upregulated and 58 downregulated genes overlapped between them. Terms of regulation of actin cytoskeleton enriched by downregulated genes and oxidative phosphorylation enriched by upregulated genes were found in 2 types of VTE. Leukocyte transendothelial migration and Jak-STAT signaling pathway were found related with recurrent VTE. In addition, genes including signal transducer and activator of transcription 3 (STAT3) involving in the Jak-STAT signaling pathway were highly connected nodes. CONCLUSIONS: Actin cytoskeleton and oxidative phosphorylation may be involved in the common mechanisms of recurrent VTE and single VTE. Leukocyte migration and Jak-STAT signaling pathway and their related genes may be important for the development and recurrence of VTE.
BACKGROUND: Unprovoked venous thromboembolism (VTE), generally divided into single and recurrent categories, is a common leading cause of morbidity and mortality in a real-world population. This study was aimed to explore the similarities and differences in the mechanisms of single and recurrent VTE. METHODS: Gene expression data (GSE19151) generated from 63 healthy controls, 32 single, and 38 recurrent VTEpatients were analyzed. Differentially expressed genes (DEGs) were screened by Affy package and Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analysis were performed using database for annotation, visualization, and integrated discovery. Based on the Search Tool for the Retrieval of Interacting Genes/Proteins database, protein-protein interaction network was visualized by Cytoscape, and modules were identified by CFinder. Finally, transcription factor regulatory networks were constructed. RESULTS: Totally, 559 and 294 DEGs were obtained from recurrent and single VTE, respectively. There were 202 upregulated and 58 downregulated genes overlapped between them. Terms of regulation of actin cytoskeleton enriched by downregulated genes and oxidative phosphorylation enriched by upregulated genes were found in 2 types of VTE. Leukocyte transendothelial migration and Jak-STAT signaling pathway were found related with recurrent VTE. In addition, genes including signal transducer and activator of transcription 3 (STAT3) involving in the Jak-STAT signaling pathway were highly connected nodes. CONCLUSIONS: Actin cytoskeleton and oxidative phosphorylation may be involved in the common mechanisms of recurrent VTE and single VTE. Leukocyte migration and Jak-STAT signaling pathway and their related genes may be important for the development and recurrence of VTE.