Malek Kammoun1, Sandra Meme2, William Meme2, Malayannan Subramaniam3, John R Hawse3, Francis Canon1, Sabine F Bensamoun1. 1. Université de Technologie de Compiègne, Centre de Recherches de Royallieu, Laboratoire de Biomécanique et de BioIngénierie, UMR CNRS 7338, BP 20529, 60205, Compiègne Cedex, France. 2. Centre de Biophysique Moléculaire, CNRS UPR4301, Orléans, France. 3. Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA.
Abstract
INTRODUCTION: Transforming growth factor-beta (TGF-β)-inducible early gene-1 (TIEG1) is a transcription factor that is highly expressed in skeletal muscle. The purpose of this study was to characterize the structural properties of both fast-twitch (EDL) and slow-twitch (soleus) muscles in the hindlimb of TIEG1-deficient (TIEG1-/- ) mice. METHODS: Ten slow and 10 fast muscles were analyzed from TIEG1-/- and wild-type (WT) mice using MRI texture (MRI-TA) and histological analyses. RESULTS: MRI-TA could discriminate between WT slow and fast muscles. Deletion of the TIEG1 gene led to changes in the texture profile within both muscle types. Specifically, muscle isolated from TIEG1-/- mice displayed hypertrophy, hyperplasia, and a modification of fiber area distribution. CONCLUSIONS: We demonstrated that TIEG1 plays an important role in the structural properties of skeletal muscle. This study further implicates important roles for TIEG1 in the development of skeletal muscle and suggests that defects in TIEG1 expression and/or function may be associated with muscle disease. Muscle Nerve 55: 410-416, 2017.
INTRODUCTION: Transforming growth factor-beta (TGF-β)-inducible early gene-1 (TIEG1) is a transcription factor that is highly expressed in skeletal muscle. The purpose of this study was to characterize the structural properties of both fast-twitch (EDL) and slow-twitch (soleus) muscles in the hindlimb of TIEG1-deficient (TIEG1-/- ) mice. METHODS: Ten slow and 10 fast muscles were analyzed from TIEG1-/- and wild-type (WT) mice using MRI texture (MRI-TA) and histological analyses. RESULTS: MRI-TA could discriminate between WT slow and fast muscles. Deletion of the TIEG1 gene led to changes in the texture profile within both muscle types. Specifically, muscle isolated from TIEG1-/- mice displayed hypertrophy, hyperplasia, and a modification of fiber area distribution. CONCLUSIONS: We demonstrated that TIEG1 plays an important role in the structural properties of skeletal muscle. This study further implicates important roles for TIEG1 in the development of skeletal muscle and suggests that defects in TIEG1 expression and/or function may be associated with muscle disease. Muscle Nerve 55: 410-416, 2017.
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Authors: T E Hefferan; G G Reinholz; D J Rickard; S A Johnsen; K M Waters; M Subramaniam; T C Spelsberg Journal: J Biol Chem Date: 2000-07-07 Impact factor: 5.157
Authors: M Subramaniam; T E Hefferan; K Tau; D Peus; M Pittelkow; S Jalal; B L Riggs; P Roche; T C Spelsberg Journal: J Cell Biochem Date: 1998-02-01 Impact factor: 4.429
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Authors: Redouane Ternifi; Malek Kammoun; Philippe Pouletaut; Malayannan Subramaniam; John R Hawse; Sabine F Bensamoun Journal: Biomed Signal Process Control Date: 2019-10-29 Impact factor: 3.880
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