Literature DB >> 27421626

Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load.

Slavica Mijatovic-Rustempasic1, Mathew D Esona1, Alice L Williams2, Michael D Bowen3.   

Abstract

Techniques such as the real-time reverse transcription-polymerase chain reaction (qRT-PCR) can detect RNA in samples with a low viral load. However, these amplicons typically are either too short or at insufficient concentrations for use in subsequent sequencing reactions for genotyping and detection confirmation. The assay developed in this study detects rotavirus G genotypes and P genotypes with viral loads as low as 6.2 and 8.2 copies per reaction, respectively. The assay was validated using a panel of 91 stool samples, 32 reference rotavirus strains, and 6 non-target enteric virus samples. Published by Elsevier B.V.

Entities:  

Keywords:  Nested; RT-PCR; Rotavirus; Sensitivity; Specificity

Mesh：

Year:  2016        PMID： 27421626      PMCID： PMC5075964          DOI： 10.1016/j.jviromet.2016.07.007

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  26 in total

1.  Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses.

Authors:  M Iturriza-Gomara; J Green; D W Brown; U Desselberger; J J Gray
Journal:  J Virol Methods       Date:  1999-03       Impact factor: 2.014

2.  Rotavirus genotyping: keeping up with an evolving population of human rotaviruses.

Authors:  Miren Iturriza-Gómara; Gagandeep Kang; Jim Gray
Journal:  J Clin Virol       Date:  2004-12       Impact factor: 3.168

3.  Detection of group B and C rotaviruses by polymerase chain reaction.

Authors:  V Gouvea; J R Allen; R I Glass; Z Y Fang; M Bremont; J Cohen; M A McCrae; L J Saif; P Sinarachatanant; E O Caul
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

Review 4.  2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis.

Authors:  Jacqueline E Tate; Anthony H Burton; Cynthia Boschi-Pinto; A Duncan Steele; Jazmin Duque; Umesh D Parashar
Journal:  Lancet Infect Dis       Date:  2011-10-24       Impact factor: 25.071

5.  Direct multiplex reverse transcription-nested PCR detection of influenza viruses without RNA purification.

Authors:  Man Ki Song; Jun Chang; Yeongjin Hong; Sunghoi Hong; Suhng Wook Kim
Journal:  J Microbiol Biotechnol       Date:  2009-11       Impact factor: 2.351

6.  Rotavirus in the home and hospital nursery.

Authors:  T H Flewett
Journal:  Br Med J (Clin Res Ed)       Date:  1983-08-27

7.  Identification of group A rotavirus gene 4 types by polymerase chain reaction.

Authors:  J R Gentsch; R I Glass; P Woods; V Gouvea; M Gorziglia; J Flores; B K Das; M K Bhan
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

8.  G and P types of circulating rotavirus strains in the United States during 1996-2005: nine years of prevaccine data.

Authors:  Jon R Gentsch; Jennifer J Hull; Elizabeth N Teel; Tara K Kerin; Molly M Freeman; Mathew D Esona; Dixie D Griffin; Brittany P Bielfelt-Krall; Krisztian Banyai; Baoming Jiang; Margaret M Cortese; Roger I Glass; Umesh D Parashar
Journal:  J Infect Dis       Date:  2009-11-01       Impact factor: 5.226

9.  Two rotavirus outbreaks caused by genotype G2P[4] at large retirement communities: cohort studies.

Authors:  Cristina V Cardemil; Margaret M Cortese; Andrew Medina-Marino; Supriya Jasuja; Rishi Desai; Jessica Leung; Cristina Rodriguez-Hart; Gissela Villarruel; Julia Howland; Osbourne Quaye; Ka Ian Tam; Michael D Bowen; Umesh D Parashar; Susan I Gerber
Journal:  Ann Intern Med       Date:  2012-11-06       Impact factor: 25.391

10.  Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG).

Authors:  Jelle Matthijnssens; Max Ciarlet; Sarah M McDonald; Houssam Attoui; Krisztián Bányai; J Rodney Brister; Javier Buesa; Mathew D Esona; Mary K Estes; Jon R Gentsch; Miren Iturriza-Gómara; Reimar Johne; Carl D Kirkwood; Vito Martella; Peter P C Mertens; Osamu Nakagomi; Viviana Parreño; Mustafizur Rahman; Franco M Ruggeri; Linda J Saif; Norma Santos; Andrej Steyer; Koki Taniguchi; John T Patton; Ulrich Desselberger; Marc Van Ranst
Journal:  Arch Virol       Date:  2011-05-20       Impact factor: 2.574
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  8 in total

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Authors:  Eric M Katz; Rashi Gautam; Michael D Bowen
Journal:  J Clin Microbiol       Date:  2017-03-08       Impact factor: 5.948

2.  Rotavirus A in wild and domestic animals from areas with environmental degradation in the Brazilian Amazon.

Authors:  Bruno de Cássio Veloso de Barros; Elaine Nunes Chagas; Luna Wanessa Bezerra; Laila Graziela Ribeiro; Jose Wandilson Barboza Duarte Júnior; Diego Pereira; Edvaldo Tavares da Penha Junior; Julia Rezende Silva; Delana Andreza Melo Bezerra; Renato Silva Bandeira; Helder Henrique Costa Pinheiro; Sylvia de Fátima Dos Santos Guerra; Ricardo José de Paula Souza E Guimarães; Joana D'Arc Pereira Mascarenhas
Journal:  PLoS One       Date:  2018-12-18       Impact factor: 3.240

3.  Fecal Source Tracking in A Wastewater Treatment and Reclamation System Using Multiple Waterborne Gastroenteritis Viruses.

Authors:  Zheng Ji; Xiaochang C Wang; Limei Xu; Chongmiao Zhang; Cheng Rong; Andri Taruna Rachmadi; Mohan Amarasiri; Satoshi Okabe; Naoyuki Funamizu; Daisuke Sano
Journal:  Pathogens       Date:  2019-09-30

Review 4.  Rotavirus Strain Trends in United States, 2009-2016: Results from the National Rotavirus Strain Surveillance System (NRSSS).

Authors:  Slavica Mijatovic-Rustempasic; Jose Jaimes; Charity Perkins; M Leanne Ward; Mathew D Esona; Rashi Gautam; Jamie Lewis; Michele Sturgeon; Junaid Panjwani; Gail A Bloom; Steve Miller; Erik Reisdorf; Ann Marie Riley; Morgan A Pence; James Dunn; Rangaraj Selvarangan; Robert C Jerris; Dona DeGroat; Umesh D Parashar; Margaret M Cortese; Michael D Bowen
Journal:  Viruses       Date:  2022-08-15       Impact factor: 5.818

5.  Rotavirus A in Domestic Pigs and Wild Boars: High Genetic Diversity and Interspecies Transmission.

Authors:  Dragan Brnić; Daniel Čolić; Valentina Kunić; Nadica Maltar-Strmečki; Nina Krešić; Dean Konjević; Miljenko Bujanić; Ivica Bačani; Dražen Hižman; Lorena Jemeršić
Journal:  Viruses       Date:  2022-09-13       Impact factor: 5.818

6.  Molecular and phylogenetic characterization of bovine coronavirus virus isolated from dairy cattle in Central Region, Thailand.

Authors:  Kanokwan Singasa; Taweesak Songserm; Preeda Lertwatcharasarakul; Pipat Arunvipas
Journal:  Trop Anim Health Prod       Date:  2017-07-18       Impact factor: 1.559

7.  Improving sensitivity of single tube nested PCR to detect fastidious microorganisms.

Authors:  Diala Shatleh-Rantisi; Asmaa Tamimi; Yaqoub Ashhab
Journal:  Heliyon       Date:  2020-01-27

8.  Ocurrence of rotavirus and picobirnavirus in wild and exotic avian from amazon forest.

Authors:  José Wandilson Barboza Duarte Júnior; Elaine Hellen Nunes Chagas; Ana Carolina Silva Serra; Lizandra Caroline Dos Santos Souto; Edvaldo Tavares da Penha Júnior; Renato da Silva Bandeira; Ricardo José de Paula Souza E Guimarães; Hanna Gabriela da Silva Oliveira; Thaymis Kiara Santos Sousa; Cinthia Távora de Albuquerque Lopes; Sheyla Farhayldes Souza Domingues; Helder Henrique Costa Pinheiro; Yashpal Singh Malik; Felipe Masiero Salvarani; Joana D'Arc Pereira Mascarenhas
Journal:  PLoS Negl Trop Dis       Date:  2021-09-10
  8 in total

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