Literature DB >> 2742156

A preparative suspension culture system permitting quantitation of anchorage-independent growth by direct radiolabeling of cellular DNA.

R K Assoian1, L A Boardman, S Drosinos.   

Abstract

We have developed a hybrid methylcellulose/agar suspension culture system which permits long-term colony formation of transformed mesenchymal cells. In contrast to traditional agar suspensions, our system allows for recovery of cells and direct biochemical analysis of anchorage-independent growth. The ability to readily radiolabel cellular macromolecules in these preparative cultures permits a quantitative and objective analysis of colony formation by incorporation of [3H]thymidine into newly synthesized DNA.

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Year:  1989        PMID： 2742156     DOI： 10.1016/0003-2697(89)90020-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Ras induces anchorage-independent growth by subverting multiple adhesion-regulated cell cycle events.

Authors:  J S Kang; R S Krauss
Journal:  Mol Cell Biol       Date:  1996-07       Impact factor: 4.272

2.  CrkII regulates focal adhesion kinase activation by making a complex with Crk-associated substrate, p130Cas.

Authors:  Toshinori Iwahara; Tsuyoshi Akagi; Yuki Fujitsuka; Hidesaburo Hanafusa
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-14       Impact factor: 11.205

3.  Activation of anchorage-independent growth of HT1080 human fibrosarcoma cells by dexamethasone.

Authors:  Nobuo Kondoh; Masahiro Shuda; Masaaki Arai; Tsuneyuki Oikawa; Mikio Yamamoto
Journal:  In Vitro Cell Dev Biol Anim       Date:  2002-02       Impact factor: 2.416

4.  Differential expression of mammalian or viral promoter-driven gene in adherent versus suspension cells.

Authors:  Gong Feng; PatriciA Hicks; Pi-Ling Chang
Journal:  In Vitro Cell Dev Biol Anim       Date:  2003 Nov-Dec       Impact factor: 2.416

5.  The lysyl oxidase pro-peptide attenuates fibronectin-mediated activation of focal adhesion kinase and p130Cas in breast cancer cells.

Authors:  Yingshe Zhao; Chengyin Min; Siddharth R Vora; Philip C Trackman; Gail E Sonenshein; Kathrin H Kirsch
Journal:  J Biol Chem       Date:  2008-11-21       Impact factor: 5.157

6.  G1/S control of anchorage-independent growth in the fibroblast cell cycle.

Authors:  T M Guadagno; R K Assoian
Journal:  J Cell Biol       Date:  1991-12       Impact factor: 10.539

7.  A cell cycle and mutational analysis of anchorage-independent growth: cell adhesion and TGF-beta 1 control G1/S transit specifically.

Authors:  E K Han; T M Guadagno; S L Dalton; R K Assoian
Journal:  J Cell Biol       Date:  1993-07       Impact factor: 10.539
  7 in total

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