Literature DB >> 2742141

Estimation of circular DNA size using gamma-irradiation and pulsed-field gel electrophoresis.

S M Beverley1.   

Abstract

A method is described for estimating the size of large circular DNAs found within complex chromosomal DNA preparations. DNAs are treated with low levels of gamma-irradiation, sufficient to introduce a single double-stranded break per circle, and the resulting linear DNA is sized by pulsed-field electrophoresis and blot hybridization. The method is fast, reproducible, and very conveniently applied to the agarose-enclosed chromosomal DNA preparations commonly used in pulsed field electrophoresis.

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Year:  1989        PMID： 2742141     DOI： 10.1016/0003-2697(89)90023-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  14 in total

1.  Asymmetric somatic cell hybridization in plants. II. Electrophoretic analysis of radiation-induced DNA damage and repair following the exposure of sugarbeet (Beta vulgaris L.) protoplasts to UV and gamma rays.

Authors:  R D Hall; G J Rouwendal; F A Krens
Journal:  Mol Gen Genet       Date:  1992-08

2.  Pulsed-field electrophoresis of megabase-sized DNA.

Authors:  K Gunderson; G Chu
Journal:  Mol Cell Biol       Date:  1991-06       Impact factor: 4.272

3.  Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates.

Authors:  R Martinez; R T Sarisky; P C Weber; S K Weller
Journal:  J Virol       Date:  1996-04       Impact factor: 5.103

4.  Stable episomal maintenance of yeast artificial chromosomes in human cells.

Authors:  K Simpson; A McGuigan; C Huxley
Journal:  Mol Cell Biol       Date:  1996-09       Impact factor: 4.272

5.  Treponema pallidum subsp. pallidum has a single, circular chromosome with a size of approximately 900 kilobase pairs.

Authors:  E M Walker; J K Arnett; J D Heath; S J Norris
Journal:  Infect Immun       Date:  1991-07       Impact factor: 3.441

6.  Exponential propagation of large circular DNA by reconstitution of a chromosome-replication cycle.

Authors:  Masayuki Su'etsugu; Hiraku Takada; Tsutomu Katayama; Hiroko Tsujimoto
Journal:  Nucleic Acids Res       Date:  2017-11-16       Impact factor: 16.971

7.  Different roles for nonhomologous end joining and homologous recombination following replication arrest in mammalian cells.

Authors:  Cecilia Lundin; Klaus Erixon; Catherine Arnaudeau; Niklas Schultz; Dag Jenssen; Mark Meuth; Thomas Helleday
Journal:  Mol Cell Biol       Date:  2002-08       Impact factor: 4.272

8.  Double targeted gene replacement for creating null mutants.

Authors:  A Cruz; C M Coburn; S M Beverley
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205

9.  PTR1: a reductase mediating salvage of oxidized pteridines and methotrexate resistance in the protozoan parasite Leishmania major.

Authors:  A R Bello; B Nare; D Freedman; L Hardy; S M Beverley
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-22       Impact factor: 11.205

10.  Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression.

Authors:  G M Kapler; C M Coburn; S M Beverley
Journal:  Mol Cell Biol       Date:  1990-03       Impact factor: 4.272
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