| Literature DB >> 2742121 |
M L Greenberg1, M S Hershfield.
Abstract
Polyethylene glycol-modified urate oxidase (PEG-uricase) holds promise as a hypouricemic agent for treating gout and as an adjunct to cytolytic therapy of hematologic malignancies. Spectrophotometric assays of urate oxidase are not sensitive enough for pharmacokinetic evaluation of PEG-uricase in clinical trials. We have therefore developed a more sensitive radiochemical-HPLC assay for urate oxidase activity in untreated plasma, in which 14C in urate and in the reaction product, allantoin, is monitored in the uv detector effluent with a flow-through scintillation counter. The assay is linear with amount of enzyme and time of incubation and can detect less than 1 x 10(-5) U/ml uricase in plasma. The assay accounts for plasma samples of widely differing urate content.Entities:
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Year: 1989 PMID： 2742121 DOI： 10.1016/0003-2697(89)90311-4
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365