Spotlight on grazoprevir-elbasvir once-daily combination and its potential in the treatment of hepatitis C.

Chronic hepatitis C virus (HCV) infection is a leading cause of health care utilization in the USA. Incidence of cirrhosis from HCV is expected to rise in the near future, further increasing this burden. There is a high medical need for effective, tolerable, safe, all-oral, short-duration therapy. To this end, several new direct-acting antiviral agents have been developed and have shown excellent sustained virologic response rates. However, patients who have previously failed treatment or who have developed cirrhosis, renal failure, or human immunodeficiency virus coinfection remain difficult-to-treat subgroups. An all-oral agent that is effective in many of these subgroups would simplify treatment of HCV greatly. Here we review currently available data on the efficacy, treatment duration, tolerability, and safety of combination of grazoprevir and elbasvir.

Introduction

Hepatitis C virus (HCV) infects an estimated 4–7 million people in the USA.1 Hepatitis C management is a burden on health care, with HCV being the most common indication for liver transplantation in the USA.2 Furthermore, the number of individuals infected with HCV who have cirrhosis in the USA is expected to rise in the future, with a peak projected between 6,00,000 and 1 million in the period between 2015 and 2020.2,3 Initial treatment options were limited to pegylated interferon and ribavirin. This regimen had sustained virologic response (SVR) rates, approximately 45%–55% at 12 weeks posttreatment.4–6 In some populations, such as the elderly and African–American patients coinfected with human immunodeficiency virus (HIV), the SVR was even lower.7–9 Furthermore, both pegylated interferon and ribavirin had high incidences of adverse events such as depression, hemolysis, pancytopenia, and live decompensation.

The introduction of direct-acting antiviral agents has revolutionized the treatment of patients infected with HCV. These new agents have resulted in dramatically improved SVR rates when compared to the previous standard of pegylated interferon and ribavirin.10,11 Previous studies have confirmed that attaining virologic cure using pegylated interferon-based therapy has resulted in significant benefits in liver-related complications and even other HCV-associated disorders such as insulin resistance.12–16 Several all-oral, direct-acting agent regimens are now available for use.

The combination of grazoprevir and elbasvir is an all-oral, direct-acting agent regimen currently being investigated for the treatment of HCV (Table 1). Grazoprevir is an NS3/4A protease inhibitor, while elbasvir is a potent NS5A inhibitor. Several Phase II and III studies have shown grazoprevir and elbasvir to be effective in previously difficult-to-treat subgroups, such as patients with cirrhosis (Child–Pugh A), previous treatment failures (null responders), coinfection with HIV, non-genotype 1, and advanced chronic kidney disease. Here we review currently available data on the efficacy, treatment duration, tolerability, and safety of grazoprevir plus elbasvir.

Table 1

Summary of SVR12 results from major clinical trials evaluating grazoprevir–elbasvir in treating HCV infection

Study	Phase	Regimen	Treatment naïve	Treatment failed	Cirrhosis	ESRD	HIV coinfection	Non-GT1
Lawitz et al17	II	GE (+R)12 weeks	28/31 (90%)	30/32 (94%)	28/31 (90%)	NA	NA	NA
		GE (−R)12 weeks	28/29 (97%)	30/33 (91%)	28/29 (97%)	NA	NA	NA
		GE (+R)18 weeks	31/32 (97%)	33/33 (100%)	12/12 (100%)	NA	NA	NA
		GE (−R)18 weeks	29/31 (94%)	31/32 (97%)	11/11 (100%)	NA	NA	NA
Sulkowski et al18	II	GE (+R)8 weeks	24/30 (80%)	NA	NA	NA	NA	NA
		GE (+R)12 weeks	79/85 (93%)	NA	NA	NA	28/29 (97%)	NA
		GE (−R)12 weeks	43/44 (98%)	NA	NA	NA	26/30 (87%)	NA
Zeuzem et al19	III	GE (−R)12 weeks	144/157 (95%)	NA	68/70 (97%)	NA	NA	GT4 18/18 (100%)GT6 8/10 (80%)
Buti et al20 and Forns et al21	II	GE (+R)12 weeks	NA	76/79 (96%)	32/34 (94%)	NA	NA	NA
Roth et al23	III	GE (−R)12 weeks	96/96 (100%)	19/20 (95%)	6/6 (100%)	115/116 (99%)	NA	NA
Rockstroh et al26	III	GE (+R)12 weeks	210/218 (96%)	NA	35/35 (100%)	NA	210/218 (96%)	GT4 27/28 (96%)GT6 1/1 (100%)

Note: Data is presented as n (%).

Abbreviations: ESRD, end-stage renal disease; GE (+R), grazoprevir–elbasvir plus ribavirin; GE (−R), grazoprevir–elbasvir without ribavirin; GT, genotype; HCV, hepatitis C virus; HIV, human immunodeficiency virus; NA, not applicable; SVR12, sustained virologic response 12 weeks posttreatment.

Treatment-naïve patients

The use of grazoprevir and elbasvir on treatment-naïve patients has been studied in three separate clinical trials. Lawitz et al17 conducted a randomized, open-label, Phase II trial (C-WORTHY) evaluating the efficacy of grazoprevir and elbasvir with and without ribavirin in patients with HCV genotype 1 who had not been previously treated for cirrhosis, as well as in patients who had previously failed pegylated interferon and ribavirin with or without cirrhosis. A total of 253 patients were involved in the study, of which 123 were treatment-naïve patients. This cohort was further divided into a 12-week course (n=60) and an 18-week course (n=63). Approximately half of the patients received ribavirin (n=64). SVR at 12 weeks posttreatment (SVR12) was achieved in 90% (28/31 patients) who underwent the 12-week regimen with ribavirin, compared to 97% (28/29 patients) without ribavirin. When treated for 18 weeks, SVR12 of 97% (31/32 patients) was achieved with ribavirin, compared to 94% (29/31 patients) without ribavirin. There was no significant benefit in the addition of ribavirin or extension of therapy to 18 weeks.

High SVR rates in treatment-naïve patients were again demonstrated in a follow-up study to the C-WORTHY trial. Sulkowski et al18 conducted a randomized, open-label, Phase II trial evaluating the efficacy of grazoprevir and elbasvir with or without ribavirin in the treatment of patients with untreated HCV genotype 1 monoinfection and patients with untreated HIV/HCV coinfection without cirrhosis. A total of 218 patients were included in the study, of which 159 patients had HCV monoinfection. SVR12 was achieved in 93% (79/85 patients) when treated for 12 weeks with ribavirin, as compared to 98% (43/44 patients) without ribavirin. There was no significant difference in SVR12 between genotypes 1a and 1b. Patients with genotype 1a who were treated for 8 weeks with ribavirin achieved an SVR12 of 80% (24/30 patients).

The efficacy of grazoprevir and elbasvir on treatment-naïve patients with different HCV genotypes has also been evaluated. Zeuzem et al19 found similar high SVR12 in treatment-naïve patients, regardless of the genotype. The C-EDGE trial was a randomized, blinded, placebo-controlled, Phase III trial looking at the efficacy of grazoprevir–elbasvir combination therapy in the treatment of previously untreated HCV genotype 1, 4, or 6 infection. A total of 421 patients with and without cirrhosis were treated for a 12-week course; no ribavirin was used in this study. Three hundred and sixteen patients received grazoprevir and elbasvir, while 105 received placebo. In the active treatment group, SVR12 was achieved in 95% (144/157) of patients. Further subgroup analysis by genotype showed an SVR12 of 92% (144/157 patients) in genotype 1a, 99% (129/131 patients) in genotype 1b, 100% (18/18 patients) in genotype 4, and 80% (8/10 patients) in genotype 6. Patients with cirrhosis achieved an SVR12 of 97% (68/70 patients), compared to 94% (231/246 patients) of noncirrhotic patients.

The above three studies showed high SVR rates with grazoprevir–elbasvir combination. There was no significant difference in SVR in treatment-naïve patients with cirrhosis, when compared to those without cirrhosis. HCV genotype did not affect SVR rates in one study. Furthermore, the use of ribavirin did not lead to increased SVR in treatment-naïve patients. Lastly, a course of 12 weeks seems to be an optimal treatment duration.

Treatment-experienced patients

Several studies examined the role of grazoprevir–elbasvir combination therapy in treatment-experienced patients, with results showing excellent SVR rates. As with treatment-naïve patients, there was no benefit with the addition of ribavirin or extension of therapy to 18 weeks. Cirrhosis again did not significantly alter SVR. Lawitz et al17 published data on patients who had previously failed pegylated interferon and ribavirin with or without cirrhosis. Of the 253 total patients included in their analysis, 130 patients had previously failed pegylated interferon and ribavirin. In a similar fashion to the treatment-naïve arm, this cohort was further divided into a 12-week course (n=65) and an 18-week course (n=65), with approximately half of the patients receiving ribavirin. SVR12 was achieved in 94% (30/32 patients) who underwent 12-week regimen with ribavirin, compared to 91% (30/33 patients) without ribavirin. When treated for 18 weeks, SVR12 of 100% (33/33 patients) was achieved with ribavirin, compared to 97% (31/32 patients) without ribavirin. SVR12 was achieved in 92% of patients with cirrhosis (23/25). There was no significant benefit with the addition of ribavirin or extension of therapy to 18 weeks.

Currently, there are many patients who have failed not only interferon-based therapies but also first-generation protease inhibitors. This subgroup of patients provides a unique challenge in treating HCV. Buti et al20 and Forns et al21 reported data on a study focusing on HCV patients who had previously failed pegylated interferon and first-generation protease inhibitors with ribavirin. The C-SALVAGE trial was an open-label, Phase II study using a 12-week course of grazoprevir plus elbasvir and ribavirin in patients with genotype 1 infection. Both cirrhotic and noncirrhotic patients were included. A total of 79 patients were enrolled in the study with the primary endpoint being evaluation of SVR12 and also SVR24. SVR12 was 96.2% (76/79 patients) with no further relapses at 24 weeks posttreatment. All three patients with virologic failure relapsed prior to 8 weeks posttreatment. Patients with cirrhosis had SVR12 of 94.1% (32/34 patients).

Advanced chronic kidney disease

Treatment of HCV in patients with advanced chronic kidney disease represents a major unmet need in hepatology.22 Roth et al23 reported data from the C-SURFER study, a randomized, placebo-controlled, Phase III study on the efficacy of grazoprevir and elbasvir in treatment-naïve and treatment-experienced patients with HCV genotype 1 infection and chronic kidney disease stage 4–5. A total of 224 patients were included in the study; 111 were assigned to the treatment group and 113 to the placebo group. An additional nonrandomized group of eleven patients underwent intensive pharmacokinetic sampling on grazoprevir and elbasvir regimen. Of the total 122 patients receiving the study drug, 116 reached the end of treatment. Six patients discontinued therapy for reasons other than virologic failure. Excluding these patients, SVR12 was achieved in 99% (115/116) of patients. Placebo reached SVR4 of <1% (1/113), with the one responder later identified as receiving the study drug incorrectly. Subgroup analysis showed excellent SVR12 in all groups receiving the study drug. Patients on hemodialysis achieved an SVR12 of 98.9% (86/87 patients), compared to 100% (29/29 patients) not on hemodialysis. Patients with stage 4 chronic kidney disease achieved an SVR12 of 100% (22/22 patients), compared to 98.9% (75/75) patients with stage 5 chronic kidney disease. In addition, there were no significant differences in SVR12 with cirrhosis, genotype 1a versus 1b, or previous treatment status.

HIV coinfection

In the USA, approximately 30% of patients with HIV are also infected with HCV.24 The major clinical implication of HIV/HCV coinfection is the faster fibrosis progression realized in coinfected relative to HCV-monoinfected patients.25 As previously mentioned, Sulkowski et al18 evaluated the efficacy of grazoprevir and elbasvir with or without ribavirin in the treatment of untreated HIV/HCV coinfected patients. A total of 59 coinfected patients underwent treatment for 12 weeks. SVR12 was 97% (28/29 patients) in the ribavirin group and 87% (26/30 patients) in the no ribavirin group. There was no significant difference with the addition of ribavirin. No significant difference was seen in SVR12 rates in coinfected patients and monoinfected patients in this study.

Grazoprevir plus elbasvir has also been shown to be effective in HIV coinfection in non-genotype 1 HCV. Rockstroh et al26 conducted an uncontrolled, nonrandomized, open-label, single-arm, Phase III study evaluating the efficacy of grazoprevir and elbasvir in patients with untreated HCV genotype 1, 4, or 6 infection and HIV coinfection with or without cirrhosis. The C-EDGE study enrolled 218 patients, with all patients receiving grazoprevir and elbasvir for 12 weeks. SVR12 was achieved in 96% (210/218) patients. Genotype 1a patients achieved an SVR12 of 96.5% (139/144 patients), compared to 95.5% (42/44 patients) in genotype 1b, 96.4% (27/28 patients) in genotype 4, and 100% (2/2 patients) in genotype 6. All 35 patients with cirrhosis achieved SVR12.

Virologic failure and resistance

All studies evaluating the efficacy of grazoprevir and elbasvir in the treatment also assessed for the presence of resistance-associated variants (RAVs) (Tables 2 and 3). NS3 and NS5A genes were amplified using reverse transcriptase-polymerase chain reaction followed by population sequencing. One of the consistent findings of these studies was the emergence of RAVs in patients who fail to respond to antiviral therapy. For instance, Lawitz et al17 evaluated baseline HCV RNA sequences for RAVs and found that 32% (79/248) had NS3 RAVs. The SVR12 in these patients was 92% (73/79 patients), compared to 96% (163/169 patients) in wild-type patients (P=0.167). The presence of NS5A RAVs was noted in 14% patients (34/243). The SVR12 was 82% (28/34) in these patients, compared to 97% (203/209 patients) in wild-type patients (P<0.001). Eight of the ten patients with virologic failure had RAVs to NS3 or NS5A and seven did not receive ribavirin. Sulkowski et al18 reported treatment failure of 4% (7/188 patients) in monoinfected and coinfected patients who were treated for 12 weeks with or without ribavirin. In contrast, the 8-week regimen plus ribavirin group of patients with HCV genotype 1a had a failure rate of 17% (5/30 patients). While there was no significant difference in virologic failure between monoinfected and HIV-coinfected patients, the authors did note that at viral loads of >10 million IU/mL, SVR12 for monoinfected patients was 90% (18/20 patients) as compared to 75% (12/16 patients), suggesting a possible difference, though not statistically significant. Baseline HCV RNA sequencing showed that NS3 RAVs were present in 35% (75/216) patients. The SVR12 in these patients was 91% (68/75 patients), as compared to 92% (130/141 patients) with wild-type NS3 (P=0.698). NS5A RAVs were found in 12% (25/216 patients) patients. Only 68% (17/25) patients in this group achieved SVR12, as compared to 95% (181/191 patients) with wild-type NS5A (P≤0.001).

Table 2

Baseline RAVs and SVR12 of varying HCV genotypes

Study	RAV	GT1	GT1a	GT1b	GT4	GT6	SVR12
Lawitz et al17	NS3	79/248 (32%)	NA	NA	NA	NA	73/79 (92%)
	NS5A	34/243 (14%)	NA	NA	NA	NA	28/34 (82%)
Sulkowski et al18	NS3	75/216 (35%)	NA	NA	NA	NA	68/75 (91%)
	NS5A	25/216 (12%)	NA	NA	NA	NA	17/25 (68%)
Zeuzem et al19	NS3	NA	86/151 (57%)	25/129 (19%)	7/18 (39%)	9/9 (100%)	GT1A: 83/86 (97%)GT1B: 24/25 (96%)GT4: 7/7 (100%)GT6: 7/9 (78%)
	NS5A	NA	19/154 (12%)	18/130 (14%)	9/18 (50%)	3/9 (33%)	GT1A: 11/19 (58%)GT1B: 17/18 (94%)GT4: 9/9 (100%)GT6: 7/9 (78%)
Buti et al20 and	NS3	34/78 (44%)	NA	NA	NA	NA	31/34 (91%)
Forns et al21	NS5A	8/79 (10)	NA	NA	NA	NA	6/8 (75%)
Roth et al23	NS3	36/112 (32%)	NA	NA	NA	NA	36/36 (100%)
	NS5A	17/115 (15%)	NA	NA	NA	NA	16/17 (94%)
Rockstroh et al26	NS3	NA	50% (69/139)	12% (5/43)	NA	NA	GT1A: 66/69 (96%)GT1B: 5/5 (100%)
	NS5A	NA	10/140 (7%)	5/43 (12%)	NA	NA	GT1A: 8/10 (80%)GT1B: 5/5 (100%)

Abbreviations: GT, genotype; HCV, hepatitis C virus; NA, not applicable; RAV, resistance-associated variant; SVR12, sustained virologic response 12 weeks posttreatment.

Table 3

Emergent RAVs posttreatment in virologic failure patients

Study	RAVs in failure patients (%)	NS3 RAVs (%)	NS5A RAVs (%)	Common NS3	Common NS5A
Lawitz et al17	9/10 (90)	7/9 (78)	9/9 (100)	Y56HA156T/G/VD168A/Y	M28TQ30L/RL31MY93H/N
Sulkowski et al18	10/12 (83)	9/10 (90)	9/10 (90)	Y56HA156TD168A/N	Q30R/HL31MY93H/N
Zeuzem et al19	13/13 (100)	9/13	13/13 (100)	Y56HD168AQ80K	M28V/A/GQ30H/L/RL31MY93H
Buti et al20 and Forns et al21	3/3 (100)	3/3 (100)	2/3 (67)	D168NQ80KA156T/A	Y93HQ30HL31M
Rockstroh et al26	3/7 (43)	2/7 (29)	4/7 (57)	Q80KD168A	Q30KY93SL31ML28S

Abbreviation: RAV, resistance-associated variant.

Differences in baseline RAVs were noted when comparing different HCV genotype subtypes. Baseline NS3 RAVs are more common than NS5A RAVs. In general, genotype 1b patients had lower incidence of NS3 RAVs at baseline, compared to genotype 1a patients (Table 2). Baseline NS5A RAV rates are similar for both genotype 1a and 1b patients. Patients with NS3 RAVs achieve SVR12 at a higher rate than patients with baseline NS5A RAVs (Table 2). For example, Zeuzem et al19 reported that baseline HCV RNA sequencing detected NS3 RAVs at baseline in 86/151 (57%) patients with genotype 1a and 25/129 (19%) patients with genotype 1b. SVR12 was achieved in 97% (83/86) genotype 1a patients with NS3 RAVs, compared to 89% (58/65) patients without RAVs. In genotype 1b patients, SVR12 was achieved in 96% (24/25) patients with NS3 RAVs and 100% (104/104) patients without RAVs. NS5A RAVs were found in 19/154 (12%) patients with genotype 1a and 18/130 (14%) patients with genotype 1b. At the conclusion of the study, SVR12 was achieved in 58% (11/19) of genotype 1a patients with NS5A RAVs, compared to 99% (133/135) without RAVs. Among genotype 1b patients, SVR12 was achieved in 94% (17/18) patients with NS5A RAVs and 100% (112/112) patients without RAVs. Among genotype 4 patients, NS3 RAVs were present in 39% (7/18), NS5A RAVs in 50% (9/18), and both were present in 11% (2/18) patients. All these patients achieved SVR12. All genotype 6 patients had NS3 RAVs and 3/9 (33%) had NS5A RAVs with SVR12 of 78% (7/9 patients) having been achieved in this group. The virologic failure rate was 4% (13 patients). At the time of virologic failure, NS3 RAVs were detected in 6/10, NS5A in 10/10, and both in 6/10 patients.

In the chronic kidney disease population, Roth et al23 reported only one virologic failure. The patient had chronic cirrhosis with genotype 1b infection and stage 5 chronic kidney disease with relapse 12 weeks after the end of treatment. Baseline NS3 or NS5A RAVs were detected in 32.1% (36/112) and 14.8% (17/115) patients, respectively. SVR was achieved in all patients with NS3 RAVs, and an SVR12 of 94.1% (16/17) was achieved in patients with NS5A RAVs.

When looking at HIV-coinfected patients, baseline NS3 and NS5A RAVs were found at similar rates. These patients also achieved SVR12 at similar rates to those of monoinfected HCV patients. Rockstroh et al26 found that baseline NS3 RAVs were present in 50% (69/139) patients with HCV genotype 1a and 12% (5/43) patients with genotype 1b. Patients with NS3 RAVs and genotype 1a achieved an SVR12 of 96% (66/69), compared to 97% (68/70) achieved with wild-type NS3. Patients with NS3 RAVs and genotype 1b achieved an SVR12 of 100% (5/5), compared to 97% (37/38) achieved with wild-type NS3. Baseline NS5A RAVs were found in 8% (15/183) patients with HCV genotype 1. These patients achieved an SVR12 of 87%, as compared to 98% (164/168) achieved in patients without NS5A RAVs. Of the eight virologic failures in this study, four were of genotype 1a and were assessed for treatment-emergent mutations. Two patients developed mutations in the NS3 region, while three had mutations in the NS5A region. One virologic failure patient was genotype 4 and had developed only an NS5A mutation.

In patients who had previously failed pegylated interferon, ribavirin, and first-generation protease inhibitors, Buti et al20 and Forns et al21 reported three (3.8%) virologic failures. All three virologic failures in this study had NS3 RAVs at baseline and two had NS5A RAVs. At baseline, 43.6% (34/78) had NS3 variants resistant to boceprevir, telaprevir, or simeprevir. An SVR12 of 91.2% (31/34 patients) was achieved in this group, compared to 100% (44/44) in patients without NS3 RAVs. NS5A RAVs were found in 10.1% (8/79) patients. SVR12 was achieved in six of these eight patients (75%).

Adverse events

Grazoprevir plus elbasvir regimen is generally well tolerated. Although drug-related adverse events may be common, serious events are infrequent. In treatment-naïve patients, Lawitz et al17 reported that of the 253 patients included in their study, 159 (63%) had a drug-related adverse event. The most common side effects reported were fatigue, headache, and asthenia. Serious adverse events occurred in seven (3%) of the patients, with three (1%) patients discontinuing treatment because of adverse events. Subgroup analysis showed higher rates of drug-related adverse events in patients given ribavirin, with 71% of patients having drug-related adverse events as compared to 54% in patients who did not receive ribavirin. All three patients who had discontinued therapy were in the ribavirin group. Sulkowski et al18 reported a drug-related adverse event rate of 56% (123/218 patients). Common adverse events included fatigue, headache, nausea, and diarrhea. Three (1%) serious adverse events were noted (one case of nausea, one case of asthenia related to the study drug, and a case of staphylococcal infection not related to the study drug). No patients died or discontinued treatment due to adverse events. Frequency of drug-related adverse events was higher (63%) in patients who received ribavirin (90/144 patients), compared to 45% (33/74 patients) in patients who did not receive ribavirin.

Two studies compared the safety profile of grazoprevir and elbasvir to that of placebo. Zeuzem et al19 reported similar safety profiles of grazoprevir and elbasvir to that of their placebo group in treatment-naïve patients. In the active treatment group, drug-related adverse events were documented as 36.1% (114/316), compared to 39% (41/105) in the placebo group. The most common adverse events in the active treatment group were headache (17%), fatigue (15%), and nausea (9%). Treatment was discontinued in three (0.9%) treatment arm patients; two patients stopped at week 8 and 12 due to elevated aminotransferase levels and one due to palpitation and anxiety on day 4 of treatment. Aminotransferase levels resolved rapidly after cessation of the study drug and SV12 was achieved in both patients. Two patients in the treatment group died, but neither was considered drug related. In patients with end-stage renal disease, Roth et al23 also reported similar safety profiles between grazoprevir and elbasvir, and placebo. Adverse event rate for study drug group was 76%, compared to 84% in the placebo group. The most common adverse events were headache, nausea, and fatigue, and were similar in both treatment and placebo groups. A total of 16 (14%) patients in the immediate treatment group reported serious side effects, compared to 19 (17%) in the placebo group. None of the serious adverse events were considered drug related. The authors reported that the frequencies and severities of liver function measures were comparable between the treatment and placebo groups. Frequencies of renal system adverse events were also comparable between both groups. Two patients in the treatment group had hemodialysis initiated during the study. Worsening chronic kidney disease stage was noted in four patients receiving the study drug, compared to two patients receiving placebo. There were no discontinuations due to drug-related adverse events in the treatment group. One patient died in the treatment group, which was not considered drug related.

Drug-related adverse events were similar in the HIV-coinfected population. Rockstroh et al26 reported an adverse event rate of 74% (161/218). Of this, 34% (75/218) was considered to be drug-related adverse event. As with previous studies, the most common adverse events were fatigue (13%), headache (12%), and nausea (9%). Six patients experienced serious adverse events, none of which were thought to be drug related. No patients discontinued treatment due to adverse events. Four patients had increased concentrations of hepatic enzymes during treatment, all of which normalized without the need for discontinuation of the study drug.

Drug–drug interactions

Grazoprevir is an inhibitor of HCV NS3/4A protease, an essential component of viral replication that functions in proteolytic cleavage of the HCV-encoded polyprotein.27 It is a cytochrome P450(CYP)3A4 and P-glycoprotein substrate and an inhibitor of CYP2C8, a weak inhibitor of 3A4 and UGTA1, and possible inhibitor of BCRP. Elbasvir is an inhibitor of HCV NS5A, which plays an important role in viral RNA replication and virion assembly. It is a CYP3A, P-gp substrate, and the organic anion-transporting polypeptide in vitro.

There have been several drug–drug interactions that have been studied (Table 4). Drugs that induce CYP3A4, such as efavirenz, have been shown to decrease the levels of grazoprevir and elbasvir.28,29 Drugs that inhibit CYP3A4 and organic anion-transporting polypeptide, such as lopinavir/ritonavir and rifampin, have shown to increase the levels of grazoprevir and elbasvir.30–32 The 3-hydroxy-3-methyl-glutaryl-CoA reductase inhibitor levels were increased in the presence of grazoprevir and elbasvir. This may be secondary to BCRP inhibition and CYP3A4 inhibition. Levels of both atorvastatin and pitavastatin were increased with coadministration, but this was not considered clinically significant.33 However, rosuvastatin, in particular, had significantly increased levels and providers may wish to avoid coadministration.34

Table 4

Potential serious drug–drug interactions with grazoprevir and elbasvir

Drug	Grazoprevir and elbasvir
Atzanavir/ritonavir30,31	Increased levels of all drugs, coadministration not recommended
Darunavir/ritonavir30	Increased levels of grazoprevir and elbasvir, coadministration is not recommended
Efavirenz28,29	Decreased levels of grazoprevir and elbasvir, coadministration may lead to subtherapeutic levels of grazoprevir
Lopinavir/ritonavir30	Increased levels of grazoprevir and elbasvir, coadministration is not recommended
Rosuvastatin34	Increased levels of rosuvastatin, avoid coadministration
Rifampin32	Increased levels of grazoprevir and elbasvir, coadministration is not recommended

Current approval

The combination of grazoprevir and elbasvir was recently approved by the US Food and Drug Administration (FDA) for the treatment of hepatitis C genotype 1 between 12 and 16 weeks and with and without ribavirin, depending on the genotype subtype, presence of NS5A polymorphisms, and type of previous experience.35 Grazoprevir and elbasvir with and without ribavirin was also approved for patients infected with genotype 4. Of note, the FDA warns that grazoprevir and elbasvir can cause elevation of liver enzymes to greater than five times the upper limit of normal. This was seen in approximately 1% of clinical trial patients. The FDA recommends that liver-related blood tests should be performed prior to starting therapy and at certain times during treatment. As discussed, elevated aminotransferase levels were typically seen at or after treatment week 8. The FDA further cautioned against the use of grazoprevir and elbasvir in patients with moderate or severe liver impairment.

Future

Although genotype 3 was not evaluated in the earlier-reviewed trials, recent data from the C-SWIFT trial using grazoprevir, elbasvir, and sofosbuvir to treat genotype 3 patients have shown excellent results with 100% (14/14) noncirrhotic patients and 91% (10/11) cirrhotic patients achieving SVR12.36 Furthermore, data from an integrated analysis of treatment-experienced patients from Phase II and III trials showed several predictors of response to grazoprevir and elbasvir.37 Among genotype 1a patients, noncirrhotics and females tended to have higher SVR12 rates. The addition of ribavirin and/or longer duration had a positive impact on SVR12, though the individual studies found no significant differences. Further studies need to be conducted to elucidate the benefits of ribavirin and longer treatment durations. Baseline NS5A RAVs had a modest negative impact on SVR12. No potential predictors were identified for genotype 1b patients.

Conclusion

Studies have shown that across many subgroups, grazoprevir–elbasvir combination has high efficacy in the treatment of HCV. High SVR12 rates were achieved regardless of the baseline factors such as genotype, cirrhosis, previous treatment failure, HIV coinfection, and renal failure. Studies comparing 12-week treatment regimens to 18-week regimens have concluded that there is no significant difference in SVR12 of the treatment regimens. Furthermore, data from currently published studies do not show a significant increase in SVR12 with the addition of ribavirin and, in fact, it has been shown to increase adverse events. Several studies have shown that ribavirin-free grazoprevir and elbasvir regimens have a good safety profile, with some studies showing no difference in adverse events when compared to placebo.