N Türkmen İnanır1, F Eren2, S Akgöz3, B Eren2, S Çetin4, U N Gündoğmuş5, N Çomunoğlu6, C Çomunoğlu7. 1. Forensic Medicine Department, Uludağ University Medical Faculty, Council of Forensic Medicine of Turkey, Bursa Morgue Department, Bursa, Turkey. 2. Bursa Morgue Department, Council of Forensic Medicine of Turkey, Bursa, Turkey. 3. Biostatistics Department, Çanakkale Onsekizmart University Medical Faculty, Çanakkale, Turkey. 4. Kayseri Morgue Department, Council of Forensic Medicine of Turkey, Kayseri, Turkey. 5. Forensic Medicine Institute, Istanbul University, Council of Forensic Medicine of Turkey, Istanbul, Turkey. 6. Pathology Department, Istanbul University Cerrahpaşa Medical Faculty, Istanbul, Turkey. 7. Pathology Department, Acıbadem University Medical Faculty, Istanbul, Turkey.
Abstract
BACKGROUND/AIM: Iron is an essential element involved in many metabolic processes. Presence and accumulation of iron in various body systems can result in different outcomes. Its accumulation in the central nervous system (CNS) cannot be detected routinely by application of hematoxylin-eosin staining. Detection of the presence of hemosiderin in the brain and cerebellum by application of Perls' dye is of importance in cases of infant deaths. MATERIAL AND METHODS: In this study, brain and cerebellar specimens obtained from 52 eligible infants (aged 0-1 years) autopsied in our institute between the years 2010 and 2013, independent of the cause of death, were analyzed in order to detect possible presence of hemosiderin. Perls' dye was used to detect histopathological staining intensity and distribution of hemosiderin in the brain and cerebellum. RESULTS: Cases did not differ significantly as for the patients' age and gender (p =0.473), type of the culprit trauma (p =0.414), death/crime scene (p =0.587), and diagnosis groups (p =0.550). In this autopsy study blue colored hemosiderin granulations, stained with Perls' dye were detected in the brain (n: 39, 75%), and cerebellum (n: 35, 67.3%). A weakly negative, but significant correlation was detected between the postmortem interval and intensity values of cerebellar hemosiderin (Spearman's correlation coefficient: -0.381, p =0.024). A statistically significant difference was found between the distribution scores of cerebral hemosiderin in cases with and without trauma history (p =0.03). Median cerebral hemosiderin distribution scores were 2.5 and 2, respectively. CONCLUSIONS: The detection of a correlation between the presence of cerebral and cerebellar hemosiderin, and postmortem interval in the age group of 0-1 years, should be interpreted as an important finding in the analysis of cerebral iron. The presence of hemosiderin in the CNS may be a significant finding in the elucidation of infant deaths and this procedure should be carried out on a routine basis.Hippokratia 2015; 19 (2):164-171.
BACKGROUND/AIM: Iron is an essential element involved in many metabolic processes. Presence and accumulation of iron in various body systems can result in different outcomes. Its accumulation in the central nervous system (CNS) cannot be detected routinely by application of hematoxylin-eosin staining. Detection of the presence of hemosiderin in the brain and cerebellum by application of Perls' dye is of importance in cases of infant deaths. MATERIAL AND METHODS: In this study, brain and cerebellar specimens obtained from 52 eligible infants (aged 0-1 years) autopsied in our institute between the years 2010 and 2013, independent of the cause of death, were analyzed in order to detect possible presence of hemosiderin. Perls' dye was used to detect histopathological staining intensity and distribution of hemosiderin in the brain and cerebellum. RESULTS: Cases did not differ significantly as for the patients' age and gender (p =0.473), type of the culprit trauma (p =0.414), death/crime scene (p =0.587), and diagnosis groups (p =0.550). In this autopsy study blue colored hemosiderin granulations, stained with Perls' dye were detected in the brain (n: 39, 75%), and cerebellum (n: 35, 67.3%). A weakly negative, but significant correlation was detected between the postmortem interval and intensity values of cerebellar hemosiderin (Spearman's correlation coefficient: -0.381, p =0.024). A statistically significant difference was found between the distribution scores of cerebral hemosiderin in cases with and without trauma history (p =0.03). Median cerebral hemosiderin distribution scores were 2.5 and 2, respectively. CONCLUSIONS: The detection of a correlation between the presence of cerebral and cerebellar hemosiderin, and postmortem interval in the age group of 0-1 years, should be interpreted as an important finding in the analysis of cerebral iron. The presence of hemosiderin in the CNS may be a significant finding in the elucidation of infant deaths and this procedure should be carried out on a routine basis.Hippokratia 2015; 19 (2):164-171.
Authors: Ashajyothi M Siddappa; Raghavendra Rao; Jeffrey D Long; John A Widness; Michael K Georgieff Journal: Neonatology Date: 2007-03-14 Impact factor: 4.035
Authors: Arnulf H Koeppen; R Liane Ramirez; Devin Yu; Sarah E Collins; Jiang Qian; Patrick J Parsons; Karl X Yang; Zewu Chen; Joseph E Mazurkiewicz; Paul J Feustel Journal: Cerebellum Date: 2012-12 Impact factor: 3.847