Literature DB >> 27418291

Optimization of PEG-based extraction of polysaccharides from Dendrobium nobile Lindl. and bioactivity study.

Yi Zhang1, Hongxin Wang2, Peng Wang1, ChaoYang Ma1, GuoHua He3, Md Ramim Tanver Rahman1.   

Abstract

Polyethylene glycol (PEG) as a green solvent was employed to extract polysaccharide. The optimal conditions for PEG-based ultrasonic extraction of Dendrobium nobile Lindl. polysaccharide (JCP) were determined by response surface methodology. Under the optimal conditions: extraction temperature of 58.5°C; ultrasound power of 193W, and the concentration of polyethylene glycol-200 (PEG-200) solution of 45%, the highest JCP yield was obtained as 15.23±0.57%, which was close to the predicted yield, 15.57%. UV and FT-IR analysis revealed the general characteristic absorption peaks of both JCP with water extraction (JCPw) and PEG-200 solvent extraction (JCPp). Thermal analysis of both JCPs was performed with Thermal Gravimetric Analyzer (TGA) and Differential Scanning Calorimeter (DSC). Antioxidant activities of two polysaccharides were also compared and no significant difference in vitro was obtained.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Antioxidant activity; Dendrobium nobile; Optimization; PEG; Polysaccharide; Response surface methodology

Mesh:

Substances:

Year:  2016        PMID: 27418291     DOI: 10.1016/j.ijbiomac.2016.07.034

Source DB:  PubMed          Journal:  Int J Biol Macromol        ISSN: 0141-8130            Impact factor:   6.953


  10 in total

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  10 in total

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