Literature DB >> 27417730

Synthesis and study on activity in vitro of the high purity human butyrylcholinesterase conjugated with gold nanoparticles.

O I Sokolov1, N Y Selivanov2, V A Bogatyrev2, O G Selivanova2, Y I Velikorodnaya3, A Y Pocheptsov3, B N Filatov3, S Y Shchyogolev2, L A Dykman2.   

Abstract

The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanoparticles was carried out by citrated method. A method of conjugation of the high-purity butyrylcholinesterase with gold nanoparticles was developed. It was found that the Immobilization of butyrylcholinesterase on the surface of gold nanoparticles resulted in a significant (to 23%) increase in the specific activity of the enzyme.

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Year:  2016        PMID： 27417730     DOI： 10.1134/S1607672916030212

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN： 1607-6729            Impact factor:   0.788


  10 in total

1.  Plant-derived human butyrylcholinesterase, but not an organophosphorous-compound hydrolyzing variant thereof, protects rodents against nerve agents.

Authors:  Brian C Geyer; Latha Kannan; Pierre-Emmanuel Garnaud; Clarence A Broomfield; C Linn Cadieux; Irene Cherni; Sean M Hodgins; Shane A Kasten; Karli Kelley; Jacquelyn Kilbourne; Zeke P Oliver; Tamara C Otto; Ian Puffenberger; Tony E Reeves; Neil Robbins; Ryan R Woods; Hermona Soreq; David E Lenz; Douglas M Cerasoli; Tsafrir S Mor
Journal:  Proc Natl Acad Sci U S A       Date:  2010-11-08       Impact factor: 11.205

Review 2.  Increasing the activity of immobilized enzymes with nanoparticle conjugation.

Authors:  Shaowei Ding; Allison A Cargill; Igor L Medintz; Jonathan C Claussen
Journal:  Curr Opin Biotechnol       Date:  2015-05-25       Impact factor: 9.740

3.  Comparison of polyethylene glycol-conjugated recombinant human acetylcholinesterase and serum human butyrylcholinesterase as bioscavengers of organophosphate compounds.

Authors:  Ofer Cohen; Chanoch Kronman; Lily Raveh; Ohad Mazor; Arie Ordentlich; Avigdor Shafferman
Journal:  Mol Pharmacol       Date:  2006-06-26       Impact factor: 4.436

Review 4.  Review of human butyrylcholinesterase structure, function, genetic variants, history of use in the clinic, and potential therapeutic uses.

Authors:  Oksana Lockridge
Journal:  Pharmacol Ther       Date:  2014-11-20       Impact factor: 12.310

5.  Butyrylcholinesterase as a therapeutic drug for protection against percutaneous VX.

Authors:  David E Lenz; Edward D Clarkson; Susan M Schulz; Douglas M Cerasoli
Journal:  Chem Biol Interact       Date:  2010-06-01       Impact factor: 5.192

6.  Kinetic study of the thermal inactivation of cholinesterase enzymes immobilized in solid matrices.

Authors:  Avraham Bromberg; Sharon Marx; Gad Frishman
Journal:  Biochim Biophys Acta       Date:  2008-03-13

7.  Effect of polyethylene glycol modification on the circulatory stability and immunogenicity of recombinant human butyrylcholinesterase.

Authors:  Nageswararao Chilukuri; Wei Sun; Ramachandra S Naik; Kalpana Parikh; Lin Tang; Bhupendra P Doctor; Ashima Saxena
Journal:  Chem Biol Interact       Date:  2008-05-21       Impact factor: 5.192

8.  Visualization of exogenous delivery of nanoformulated butyrylcholinesterase to the central nervous system.

Authors:  Andrea Gaydess; Ellen Duysen; Yuan Li; Vladimir Gilman; Alexander Kabanov; Oksana Lockridge; Tatiana Bronich
Journal:  Chem Biol Interact       Date:  2010-01-11       Impact factor: 5.192

9.  Substantially improved pharmacokinetics of recombinant human butyrylcholinesterase by fusion to human serum albumin.

Authors:  Yue-Jin Huang; Paul M Lundy; Anthoula Lazaris; Yue Huang; Hernan Baldassarre; Bin Wang; Carl Turcotte; Mélanie Côté; Annie Bellemare; Annie S Bilodeau; Sandra Brouillard; Madjid Touati; Peter Herskovits; Isabelle Bégin; Nathalie Neveu; Eric Brochu; Janice Pierson; Duncan K Hockley; Douglas M Cerasoli; David E Lenz; Harvey Wilgus; Costas N Karatzas; Solomon Langermann
Journal:  BMC Biotechnol       Date:  2008-05-16       Impact factor: 2.563

10.  Chemical Polysialylation and In Vivo Tetramerization Improve Pharmacokinetic Characteristics of Recombinant Human Butyrylcholinesterase-Based Bioscavengers.

Authors:  S S Terekhov; I V Smirnov; O G Shamborant; T V Bobik; D G Ilyushin; A N Murashev; I A Dyachenko; V A Palikov; V D Knorre; A A Belogurov; N A Ponomarenko; E S Kuzina; D D Genkin; P Masson; A G Gabibov
Journal:  Acta Naturae       Date:  2015 Oct-Dec       Impact factor: 1.845
  10 in total
  2 in total

Review 1.  Cholinesterases and the fine line between poison and remedy.

Authors:  Carey N Pope; Stephen Brimijoin
Journal:  Biochem Pharmacol       Date:  2018-01-31       Impact factor: 5.858

2.  Doxorubicin-fucoidan-gold nanoparticles composite for dual-chemo-photothermal treatment on eye tumors.

Authors:  Hyejin Kim; Van Phuc Nguyen; Panchanathan Manivasagan; Min Jung Jung; Sung Won Kim; Junghwan Oh; Hyun Wook Kang
Journal:  Oncotarget       Date:  2017-12-09
  2 in total

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