| Literature DB >> 27411009 |
Dorien Reijnders1, Gijs H Goossens1, Gerben D A Hermes2, Evelien P J G Neis3, Christina M van der Beek3, Jasper Most4, Jens J Holst5, Kaatje Lenaerts3, Ruud S Kootte6, Max Nieuwdorp6, Albert K Groen7, Steven W M Olde Damink8, Mark V Boekschoten9, Hauke Smidt2, Erwin G Zoetendal2, Cornelis H C Dejong3, Ellen E Blaak10.
Abstract
The gut microbiota has been implicated in obesity and cardiometabolic diseases, although evidence in humans is scarce. We investigated how gut microbiota manipulation by antibiotics (7-day administration of amoxicillin, vancomycin, or placebo) affects host metabolism in 57 obese, prediabetic men. Vancomycin, but not amoxicillin, decreased bacterial diversity and reduced Firmicutes involved in short-chain fatty acid and bile acid metabolism, concomitant with altered plasma and/or fecal metabolite concentrations. Adipose tissue gene expression of oxidative pathways was upregulated by antibiotics, whereas immune-related pathways were downregulated by vancomycin. Antibiotics did not affect tissue-specific insulin sensitivity, energy/substrate metabolism, postprandial hormones and metabolites, systemic inflammation, gut permeability, and adipocyte size. Importantly, energy harvest, adipocyte size, and whole-body insulin sensitivity were not altered at 8-week follow-up, despite a still considerably altered microbial composition, indicating that interference with adult microbiota by 7-day antibiotic treatment has no clinically relevant impact on metabolic health in obese humans.Entities:
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Year: 2016 PMID： 27411009 DOI： 10.1016/j.cmet.2016.06.016
Source DB: PubMed Journal: Cell Metab ISSN： 1550-4131 Impact factor: 27.287