Jessica M Garcia-Vivas1, Carlos Galaviz-Hernandez2, Jorge Fernandez-Retana3, Abraham Pedroza-Torres3, Carlos Perez-Plasencia3, Cesar Lopez-Camarillo4, Laurence A Marchat1,5. 1. 1 Biotechnology Program, ENMH, Instituto Politécnico Nacional , Mexico City, Mexico . 2. 2 Biotechnology Program, CIIDIR, Instituto Politécnico Nacional , Durango City, Durango, Mexico . 3. 3 Oncogenomics Laboratory, National Institute of Cancerology , Mexico City, Mexico . 4. 4 Genomics Sciences Program, Universidad Autónoma de la Ciudad de México , Mexico City, Mexico . 5. 5 Molecular Biomedicine Program, ENMH, Instituto Politécnico Nacional , Mexico City, Mexico .
Abstract
OBJECTIVE: Complementary and alternative medicine, such as Traditional Chinese Medicine, represents an efficient therapeutic option for obesity control. It was previously reported that acupuncture catgut embedding therapy (ACET) with moxibustion reduces body weight and reverts insulin resistance in obese women. This study aimed to evidence changes in adipokines and gene expression in adipose tissue that could explain the effects of ACET with moxibustion. DESIGN:Overweight/obese women were treated with ACET with moxibustion or sham acupuncture as control. Peripheral blood samples and fat biopsies were taken before and after intervention. Circulating adipokines (leptin, adiponectin, tumor necrosis factor alpha, and resistin) were quantified by enzyme-linked immunosorbent assay. Gene expression in adipose tissue was determined by cDNA microarray assays and assessed by quantitative reverse transcription real-time polymerase chain reaction. RESULTS:ACET with moxibustion did not modify circulating adipokines levels. However, correlations with anthropometric and biochemical parameters were affected. Interestingly, transcriptional changes in adipose tissue revealed the modulation of genes participating in homeostasis control, lipid metabolism, olfactory transduction, and gamma-aminobutyric acid signaling pathway. CONCLUSIONS: The effects of ACET with moxibustion on body weight and insulin resistance were associated with the regulation of biochemical events that are altered in obesity.
RCT Entities:
OBJECTIVE: Complementary and alternative medicine, such as Traditional Chinese Medicine, represents an efficient therapeutic option for obesity control. It was previously reported that acupuncture catgut embedding therapy (ACET) with moxibustion reduces body weight and reverts insulin resistance in obesewomen. This study aimed to evidence changes in adipokines and gene expression in adipose tissue that could explain the effects of ACET with moxibustion. DESIGN: Overweight/obesewomen were treated with ACET with moxibustion or sham acupuncture as control. Peripheral blood samples and fat biopsies were taken before and after intervention. Circulating adipokines (leptin, adiponectin, tumor necrosis factor alpha, and resistin) were quantified by enzyme-linked immunosorbent assay. Gene expression in adipose tissue was determined by cDNA microarray assays and assessed by quantitative reverse transcription real-time polymerase chain reaction. RESULTS:ACET with moxibustion did not modify circulating adipokines levels. However, correlations with anthropometric and biochemical parameters were affected. Interestingly, transcriptional changes in adipose tissue revealed the modulation of genes participating in homeostasis control, lipid metabolism, olfactory transduction, and gamma-aminobutyric acid signaling pathway. CONCLUSIONS: The effects of ACET with moxibustion on body weight and insulin resistance were associated with the regulation of biochemical events that are altered in obesity.
Authors: Jae Ik Kim; Young Il Kim; Eunseok Kim; Ju Hyun Jeon; Jin Youp Kim; Ojin Kwon; So-Young Jung; Chang-Hyun Han Journal: Medicine (Baltimore) Date: 2019-08 Impact factor: 1.817
Authors: Jae Ik Kim; Chang-Hyun Han; Ju Hyun Jeon; Jin Youp Kim; Ojin Kwon; So-Young Jung; Jun-Hwan Lee; Changsop Yang; Eunseok Kim; Young Il Kim Journal: J Pain Res Date: 2021-01-28 Impact factor: 3.133