| Literature DB >> 27402482 |
E S Ershova1, V A Sergeeva2, A I Chausheva2, D G Zheglo2, V A Nikitina2, T D Smirnova2, L V Kameneva2, L N Porokhovnik3, S I Kutsev4, P A Troshin5, I I Voronov5, E A Khakina5, N N Veiko1, S V Kostyuk2.
Abstract
Water-soluble fullerenes have been studied as potential nanovectors and therapeutic agents, but their possible toxicity is of concern. We have studied the effects of F-828, a soluble fullerene [C60] derivative, on diploid human embryonic lung fibroblasts (HELFs) in vitro. F-828 causes complex time-dependent changes in ROS levels. Inhibition of Nox4 activity by plumbagin blocks F-828-dependent ROS elevation. F-828 induces DNA breaks, as measured by the comet assay and γH2AX expression, and the activities of the transcription factors NF-kB and p53 increase. F-828 concentrations>25μM are cytotoxic; cell death occurs by necrosis. Expression levels of TGF-β, RHOA, RHOC, ROCK1, and SMAD2 increase following exposure to F-828. Our results raise the possibility that fullerene F-828 may induce pulmonary fibrosis in vivo.Entities:
Keywords: Comet assay; DNA damage; Fibroblasts; Free radicals; Fullerene
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Year: 2016 PMID： 27402482 DOI： 10.1016/j.mrgentox.2016.05.004
Source DB: PubMed Journal: Mutat Res Genet Toxicol Environ Mutagen ISSN： 1383-5718 Impact factor: 2.873