| Literature DB >> 27401074 |
Wei Ji1, Hanzhang Liu2, Chun Liu3, Lifei Shao1, Yuankun Liu1, Shaochen Fan1, Xiaohong Li4, Lei Lei Gong5, Shunxing Zhu6, Yilu Gao7.
Abstract
Minichromosome maintenance complex component 3, one of the minichromosome maintenance proteins, functions as a part of pre-replication complex to initiate DNA replication in eukaryotes. Minichromosome maintenance complex component 3 (MCM3) was mainly implied in cell proliferation and tumorigenesis. In addition, MCM3 might play an important role in neuronal apoptosis. However, the functions of MCM3 in central nervous system are still with limited acquaintance. In this study, we performed a traumatic brain injury (TBI) model in adult rats. Western blot and immunohistochemistry staining showed up-regulation of MCM3 in the peritrauma brain cortex. The expression patterns of active caspase-3 and Bax, Bcl-2 were parallel with that of MCM3. Immunofluorescent staining and terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling suggested that MCM3 was involved in neuronal apoptosis. In conclusion, our data indicated that MCM3 might play an important role in neuronal apoptosis following TBI. Further understanding of these insights could serve as the basis for broadening the therapeutic scope against TBI.Entities:
Keywords: Active caspase-3; BAX/Bcl-2; MCM3; Neuronal apoptosis; TBI
Mesh：
Substances：
Year: 2016 PMID： 27401074 DOI： 10.1007/s10571-016-0404-x
Source DB: PubMed Journal: Cell Mol Neurobiol ISSN： 0272-4340 Impact factor: 5.046