| Literature DB >> 27396335 |
Charlotte D'Hulst1, Raena B Mina1, Zachary Gershon1, Sophie Jamet1, Antonio Cerullo1, Delia Tomoiaga1, Li Bai2, Leonardo Belluscio2, Matthew E Rogers3, Yevgeniy Sirotin4, Paul Feinstein5.
Abstract
Typically, ∼0.1% of the total number of olfactory sensory neurons (OSNs) in the main olfactory epithelium express the same odorant receptor (OR) in a singular fashion and their axons coalesce into homotypic glomeruli in the olfactory bulb. Here, we have dramatically increased the total number of OSNs expressing specific cloned OR coding sequences by multimerizing a 21-bp sequence encompassing the predicted homeodomain binding site sequence, TAATGA, known to be essential in OR gene choice. Singular gene choice is maintained in these "MouSensors." In vivo synaptopHluorin imaging of odor-induced responses by known M71 ligands shows functional glomerular activation in an M71 MouSensor. Moreover, a behavioral avoidance task demonstrates that specific odor detection thresholds are significantly decreased in multiple transgenic lines, expressing mouse or human ORs. We have developed a versatile platform to study gene choice and axon identity, to create biosensors with great translational potential, and to finally decode human olfaction.Entities:
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Year: 2016 PMID： 27396335 DOI： 10.1016/j.celrep.2016.06.047
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423