Chao Zhang1, Mu-Xin Gong1, Feng Qiu1, Jing Li1, Man-Yuan Wang2. 1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. 2. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. Electronic address: wangmyjun@163.com.
Abstract
OBJECTIVE: To explore the effects of arteannuin B, arteannuic acid and scopoletin on the pharmacokinetics of artemisinin in mice. METHODS: Artemisinin and a combination of artemisinin, arteannuin B, arteannuic acid and scopoletin were administered together to mice via oral administration. Blood samples were collected at different time intervals and pretreated by liquid-liquid extraction. The contents of four compounds in mouse plasma were determined by a validated HPLC-MS/MS method. RESULTS: Compared to single artemisinin group, the Cmax values from the combination group rose from 947 ng/mL to 1254 ng/mL. AUC(0-t) (2371 h ng/mL) was significantly higher than that from single artemisinin group (747 h ng/mL). The peak time lag and the CL values reduced at a proportion of 66%. CONCLUTIONS: Arteannuin B, arteannuic acid and scopoletin can markedly affect the pharmacokinetics of artemisinin.
OBJECTIVE: To explore the effects of arteannuin B, arteannuic acid and scopoletin on the pharmacokinetics of artemisinin in mice. METHODS: Artemisinin and a combination of artemisinin, arteannuin B, arteannuic acid and scopoletin were administered together to mice via oral administration. Blood samples were collected at different time intervals and pretreated by liquid-liquid extraction. The contents of four compounds in mouse plasma were determined by a validated HPLC-MS/MS method. RESULTS: Compared to single artemisinin group, the Cmax values from the combination group rose from 947 ng/mL to 1254 ng/mL. AUC(0-t) (2371 h ng/mL) was significantly higher than that from single artemisinin group (747 h ng/mL). The peak time lag and the CL values reduced at a proportion of 66%. CONCLUTIONS: Arteannuin B, arteannuic acid and scopoletin can markedly affect the pharmacokinetics of artemisinin.