Bong-Keun Choi1, Sung-Bum Park2, Dong-Ryung Lee1, Hae Jin Lee2, Ying-Yu Jin3, Seung Hwan Yang4, Joo-Won Suh5. 1. Nutra Pharm Tech, Giheung-gu, Yongin, Gyeonggi 446-916, South Korea. 2. Interdisciplinary Program of Biomodulation, Myongji University, Yongin, Gyeonggi 446-728, South Korea. 3. Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Gyeonggi 446-728, South Korea. 4. Interdisciplinary Program of Biomodulation, Myongji University, Yongin, Gyeonggi 446-728, South Korea; Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Gyeonggi 446-728, South Korea. Electronic address: ymichigan@mju.ac.kr. 5. Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Gyeonggi 446-728, South Korea. Electronic address: jwsuh@mju.ac.kr.
Abstract
OBJECTIVES: To evaluate possible lipid catabolism and body fat regulation effects of 3-caffeoylquinic acid in Green coffee bean extract (GCBE) in high-fat diet (HFD)-induced obese mice. METHODS: Obesity was induced in mice using a HFD for four weeks. Then, mice were fed only HFD or HFD with GCBE at 50, 100, and 200 mg/kg. Fatty acid synthesis mechanism regulation of body fat was investigated through real-time PCR and Western blot assay. Body fat reduction was measured through dual-energy X-ray absorptiometry. RESULTS: In HFD-induced obese mice, GCBE treatment significantly decreased body weight gain, liver weight and white adipose tissue weights with regulation of adipose tissue lipolysis hormones, like adiponectin and leptin. GCBE treatment decreased mRNA expression levels of adipogenesis and adipocyte metabolism related genes in adipose tissues and the liver, and decreased the corresponding protein expression. Dual energy X-ray absorptiometry measurements were used to compare body fat between mice on high-fat and those treated with GCBE. GCBE treated mice had a lower fat mass compared to HFD alone fed mice and relative body weight and fat mass were markedly decreased. CONCLUSIONS: GCBE has a potential anti-obesity effect with lowering body fat accumulation by regulating adipogenesis and lipid metabolism-related genes and proteins in WAT and liver.
OBJECTIVES: To evaluate possible lipid catabolism and body fat regulation effects of 3-caffeoylquinic acid in Green coffee bean extract (GCBE) in high-fat diet (HFD)-induced obesemice. METHODS:Obesity was induced in mice using a HFD for four weeks. Then, mice were fed only HFD or HFD with GCBE at 50, 100, and 200 mg/kg. Fatty acid synthesis mechanism regulation of body fat was investigated through real-time PCR and Western blot assay. Body fat reduction was measured through dual-energy X-ray absorptiometry. RESULTS: In HFD-induced obesemice, GCBE treatment significantly decreased body weight gain, liver weight and white adipose tissue weights with regulation of adipose tissue lipolysis hormones, like adiponectin and leptin. GCBE treatment decreased mRNA expression levels of adipogenesis and adipocyte metabolism related genes in adipose tissues and the liver, and decreased the corresponding protein expression. Dual energy X-ray absorptiometry measurements were used to compare body fat between mice on high-fat and those treated with GCBE. GCBE treated mice had a lower fat mass compared to HFD alone fed mice and relative body weight and fat mass were markedly decreased. CONCLUSIONS: GCBE has a potential anti-obesity effect with lowering body fat accumulation by regulating adipogenesis and lipid metabolism-related genes and proteins in WAT and liver.
Authors: Liyuan Han; Wenjie Ma; Dianjianyi Sun; Yoriko Heianza; Tiange Wang; Yan Zheng; Tao Huang; Donghui Duan; J George A Bray; Catherine M Champagne; Frank M Sacks; Lu Qi Journal: Am J Clin Nutr Date: 2017-09-20 Impact factor: 7.045