AIM: Development of liposomal formulations of Cuphen, a potent copper-based aquaporin inhibitor with therapeutic potential against melanoma and colon cancer. MATERIALS & METHODS: Cuphen was incorporated into liposomes using the dehydration-rehydration method. The ability of Cuphen to induce cancer cell death was evaluated by MTS and ViaCount assays. In vivo toxicity studies were performed in BALB/c mice. RESULTS: In vitro studies illustrated the antiproliferative effects of Cuphen in different cancer cell lines, in free form or after incorporation into liposomes. In vivo studies revealed no toxic effects after parenteral administration of Cuphen liposomes. CONCLUSIONS: Cuphen liposomes are highly attractive to be further tested in murine models due to the possibility of stabilizing and specifically deliver this metallodrug to tumor sites.
AIM: Development of liposomal formulations of Cuphen, a potent copper-based aquaporin inhibitor with therapeutic potential against melanoma and colon cancer. MATERIALS & METHODS:Cuphen was incorporated into liposomes using the dehydration-rehydration method. The ability of Cuphen to induce cancer cell death was evaluated by MTS and ViaCount assays. In vivo toxicity studies were performed in BALB/c mice. RESULTS: In vitro studies illustrated the antiproliferative effects of Cuphen in different cancer cell lines, in free form or after incorporation into liposomes. In vivo studies revealed no toxic effects after parenteral administration of Cuphen liposomes. CONCLUSIONS:Cuphen liposomes are highly attractive to be further tested in murine models due to the possibility of stabilizing and specifically deliver this metallodrug to tumor sites.
Entities:
Keywords:
Cuphen; aquaporins; colon cancer; cytotoxic effect; in vivo toxicity studies; liposomes; melanoma; metallodrugs
Authors: Harshita Mishra; Pawan K Mishra; Adam Ekielski; Manu Jaggi; Zeenat Iqbal; Sushama Talegaonkar Journal: J Cancer Res Clin Oncol Date: 2018-08-09 Impact factor: 4.553
Authors: Catarina Pimpão; Inês V da Silva; Andreia F Mósca; Jacinta O Pinho; Maria Manuela Gaspar; Nadiia I Gumerova; Annette Rompel; Manuel Aureliano; Graça Soveral Journal: Int J Mol Sci Date: 2020-04-02 Impact factor: 5.923