| Literature DB >> 27388263 |
Seiya Watanabe1,2, Kunihiko Tajima3, Kazuma Matsui3, Yasuo Watanabe1.
Abstract
Flavin-containing opine dehydrogenase from Bradyrhizobium japonicum forms a heterooligomeric α4β4γ4 enzyme complex. An electron paramagnetic resonance spectroscopy analysis using wild-type and site-directed mutants revealed that [4Fe-4S] and [2Fe-2S] clusters bind to two different types of [Fe-S] binding sites in the γ- and α-subunits, respectively. The latter was found to be important for structural folding and enzyme catalysis.Entities:
Keywords: [2Fe-2S] cluster; [4Fe-4S] cluster; electron spin resonance spectroscopy; opine dehydrogenase
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Year: 2016 PMID： 27388263 DOI： 10.1080/09168451.2016.1206812
Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN： 0916-8451 Impact factor: 2.043