| Literature DB >> 27387821 |
Derek L Davis1, Erin K Price1, Sabrina O Aderibigbe1, Maureen X-H Larkin1, Emmett D Barlow1, Renjie Chen1, Lincoln C Ford1, Zackery T Gray1, Stephen H Gren1, Yuwei Jin1, Keith S Keddington1, Alexandra D Kent1, Dasom Kim1, Ashley Lewis1, Rami S Marrouche1, Mark K O'Dair1, Daniel R Powell1, Mick'l H C Scadden1, Curtis B Session1, Jifei Tao1, Janelle Trieu1, Kristen N Whiteford1, Zheng Yuan1, Goyeun Yun1, Judy Zhu1, Jennifer M Heemstra1.
Abstract
We investigate the effect of buffer identity, ionic strength, pH, and organic cosolvents on the rate of strain-promoted azide-alkyne cycloaddition with the widely used DIBAC cyclooctyne. The rate of reaction between DIBAC and a hydrophilic azide is highly tolerant to changes in buffer conditions but is impacted by organic cosolvents. Thus, bioconjugation reactions using DIBAC can be carried out in the buffer that is most compatible with the biomolecules being labeled, but the use of organic cosolvents should be carefully considered.Entities:
Year: 2016 PMID： 27387821 DOI： 10.1021/acs.joc.6b01112
Source DB: PubMed Journal: J Org Chem ISSN： 0022-3263 Impact factor: 4.354