Eliseth Costa Oliveira de Matos1, Haroldo José de Matos2, Marília Lima Conceição3, Yan Corrêa Rodrigues3, Irna Carla do Rosário Souza Carneiro4, Karla Valéria Batista Lima2,3. 1. Núcleo de Medicina Tropical, Programa de Pós-Graduação em Medicina Tropical, Universidade Federal do Pará, Belém, Pará, Brasil. 2. Seção de Bacteriologia e Micologia, Instituto Evandro Chagas, Ministério da Saúde, Ananindeua, Pará, Brasil. 3. Programa de Pós-Graduação em Biologia Parasitária na Amazônia, Universidade do Estado do Pará, Belém, Pará, Brasil. 4. Centro de Ciências Biológicas e Saúde, Universidade do Estado do Pará, Belém, Pará, Brasil.
Abstract
INTRODUCTION: The spread of multidrug-resistant Pseudomonas aeruginosa in Brazilian hospitals has greatly impacted upon the morbidity and mortality of individuals in intensive care units. Given the lack of information regarding the dynamics of multidrug resistance in northern Brazil, we analyzed the clinical and microbiological features of nosocomial infections caused by P. aeruginosa. METHODS: Between January 2010 and March 2012, we conducted a retrospective cohort study of P. aeruginosa isolates from 54 patients who were hospitalized in intensive care units. The clinical and epidemiologic variables were analyzed, including the patients' demographic data and comorbidities, and the lengths of the intensive care unit stays, the classification of the infections as nosocomial, the use of invasive procedures, antimicrobial therapy, and the patients' outcomes. We undertook susceptibility tests, molecular detection of the metallo-β-lactamase genes, and genotypic analyses of the isolates using the repetitive element-polymerase chain reaction. RESULTS: Multidrug resistance occurred most frequently among isolates from adults who had been hospitalized for an average of 87.1 days. The use of mechanical ventilation and urinary catheters were risk factors for infection. The four isolates that harbored the blaSPM-1-like gene showed >95% genetic similarity. CONCLUSIONS: This study's findings show that P. aeruginosa has a high death rate, and that inadequate treatment and invasive procedures are risk factors for infection. This is the first report describing the detection of the blaSPM-1-like gene in northern Brazil. These results highlight the need for better monitoring and a greater understanding of nosocomial infections and their public health impacts.
INTRODUCTION: The spread of multidrug-resistant Pseudomonas aeruginosa in Brazilian hospitals has greatly impacted upon the morbidity and mortality of individuals in intensive care units. Given the lack of information regarding the dynamics of multidrug resistance in northern Brazil, we analyzed the clinical and microbiological features of nosocomial infections caused by P. aeruginosa. METHODS: Between January 2010 and March 2012, we conducted a retrospective cohort study of P. aeruginosa isolates from 54 patients who were hospitalized in intensive care units. The clinical and epidemiologic variables were analyzed, including the patients' demographic data and comorbidities, and the lengths of the intensive care unit stays, the classification of the infections as nosocomial, the use of invasive procedures, antimicrobial therapy, and the patients' outcomes. We undertook susceptibility tests, molecular detection of the metallo-β-lactamase genes, and genotypic analyses of the isolates using the repetitive element-polymerase chain reaction. RESULTS: Multidrug resistance occurred most frequently among isolates from adults who had been hospitalized for an average of 87.1 days. The use of mechanical ventilation and urinary catheters were risk factors for infection. The four isolates that harbored the blaSPM-1-like gene showed >95% genetic similarity. CONCLUSIONS: This study's findings show that P. aeruginosa has a high death rate, and that inadequate treatment and invasive procedures are risk factors for infection. This is the first report describing the detection of the blaSPM-1-like gene in northern Brazil. These results highlight the need for better monitoring and a greater understanding of nosocomial infections and their public health impacts.
Authors: Rajbharan Yadav; Jürgen B Bulitta; Elena K Schneider; Beom Soo Shin; Tony Velkov; Roger L Nation; Cornelia B Landersdorfer Journal: Antimicrob Agents Chemother Date: 2017-11-22 Impact factor: 5.191
Authors: Sandra Patricia Rivera-Sánchez; Helen Astrid Agudelo-Góngora; José Oñate-Garzón; Liliana Janeth Flórez-Elvira; Adriana Correa; Paola Andrea Londoño; Juan David Londoño-Mosquera; Alberto Aragón-Muriel; Dorian Polo-Cerón; Iván Darío Ocampo-Ibáñez Journal: Molecules Date: 2020-10-30 Impact factor: 4.411