| Literature DB >> 27384176 |
Jianxin Wang1,2, Wenxia Peng1,2, Jinrong Feng2, Dandan Zhu2, Jinling Chen2, Xiaolei Sun2, Lei Lyu2, Shaoqing Ju3, Yinong Duan4,5.
Abstract
Recombinant T2 RNase glycoprotein, which showed a certain degree of homology to Omega-1 from Schistosoma mansoni eggs, was expressed in adult worms of Schistosoma japonicum, but not in eggs of S. japonicum. The direct biological role of the recombinant T2 RNase protein in activation of hepatic stellate cells (HSCs) remains unknown. In the present study, the immortalized human HSC line (LX-2 cells) was treated with the recombinant T2 RNase protein at indicated concentrations for various time points in vitro. The expression levels of α-smooth muscle actin (α-SMA) and Smad4 were detected by Western blot. The results showed that the recombinant T2 RNase protein significantly diminished the expression levels of α-SMA and Smad4 in LX-2 cells. The upregulated expression levels of α-SMA and Smad4 by TGF-β1 in LX-2 cells were both suppressed by the recombinant T2 RNase protein. These data suggest that the recombinant T2 RNase protein may be a potential target of therapeutic strategy for the treatment of hepatic fibrosis.Entities:
Keywords: Hepatic stellate cell; Schistosoma japonicum; Smad4; T2 RNase; α-SMA
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Year: 2016 PMID： 27384176 DOI： 10.1007/s00436-016-5178-z
Source DB: PubMed Journal: Parasitol Res ISSN： 0932-0113 Impact factor: 2.289