Function and regulation of the avian caecal bulb: influence of dietary NaCl and aldosterone on water and electrolyte fluxes in the hen (Gallus domesticus) perfused in vivo.

The function of the caecal bulb, and its adaptation to chronic high- or low-Na+ intake, was investigated by in vivo perfusion of anaesthetised birds. Effects of acute aldosterone injection (125 micrograms.kg-1 body mass) were also measured. Evidence was found for primary active net absorption of Na+, inducing parallel Na-linked absorption of water and Cl- and secretion of K+. Around 20-35% of total Cl- absorption and K+ secretion were independent of Na+ fluxes, and these components appear to be driven by passive processes with apparent conductances of 6.3 X 10(-3) (GCl) and 1.1 X 10(-3) (GK) S.cm-2. Acetate (40 mM) stimulated Na+ fluxes (8.5-9.9 microEq.cm-2.h-1) and Na-linked water fluxes (27-44 microliters.cm-2.h-1). Increased coupling ratios (2.9-4.6 microliters.microEq-1) and other data indicate that these effects may be due to increased osmotic permeabilities of barriers involved in the Na-linked water transfer pathway. Low-Na+ maintenance enhanced EPD (49-69 mV, serosa positive) and all net fluxes: JNa (6.8-11.6); JK (-3.2--4.3); JCl (4.3-5.6 microEq.cm serosal area-2.h-1); Jv (28-43 microliters.cm-2.h-1) (mucosal-serosal fluxes positive). Acute aldosterone enhanced JNa (10.8-14.0 microEq.cm-2.h-1) and EPD (54-66 mV) by 3 h after injection, but had no effect on the Na-linked components of JK or JCl.