Kanae Sugano1, Kotomi Hashi2, Misaki Goto3, Kiyotaka Nishi4, Rie Maeda5, Keigo Kono6, Mai Yamamoto6, Kazunori Okada7, Sanae Kaga7, Keiko Miwa7,8, Taisei Mikami7, Nobuo Masauzi7. 1. Department of Clinical Laboratory, NTT East Japan Sapporo Hospital, Sapporo, Japan. 2. Department of Clinical Laboratory, Daiichi Kishimoto Medical Laboratory Center, Sapporo, Japan. 3. Department of Clinical Laboratory, Sapporo Hokuyu Hospital, Sapporo, Japan. 4. Department of Clinical Laboratory, Teine Keijinnkai Hospital, Sapporo, Japan. 5. Department of Clinical Laboratory, KKR Sapporo Medical Center, Sapporo, Japan. 6. Graduate school of Health Sciences, Hokkaido University, Sapporo, Japan. 7. Department of Medical laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan. 8. Department of Cardiovascular Regenerative Medicine, Osaka University Graduate School of Medicine, Suita, Japan.
Abstract
BACKGROUND: The neutrophil alkaline phosphatase (NAP) score is a valuable test for the diagnosis of myeloproliferative neoplasms, but it has still manually rated. Therefore, we developed a semi-automatic rating method using Photoshop® and Image-J, called NAP-PS-IJ. METHODS: Neutrophil alkaline phosphatase staining was conducted with Tomonaga's method to films of peripheral blood taken from three healthy volunteers. At least 30 neutrophils with NAP scores from 0 to 5+ were observed and taken their images. From which the outer part of neutrophil was removed away with Image-J. These were binarized with two different procedures (P1 and P2) using Photoshop® . NAP-positive area (NAP-PA) and granule (NAP-PGC) were measured and counted with Image-J. RESULTS: The NAP-PA in images binarized with P1 significantly (P < 0.05) differed between images with NAP scores from 0 to 3+ (group 1) and those from 4+ to 5+ (group 2). The original images in group 1 were binarized with P2. NAP-PGC of them significantly (P < 0.05) differed among all four NAP score groups. The mean NAP-PGC with NAP-PS-IJ indicated a good correlation (r = 0.92, P < 0.001) to results by human examiners. CONCLUSIONS: The sensitivity and specificity of NAP-PS-IJ were 60% and 92%, which might be considered as a prototypic method for the full-automatic rating NAP score.
BACKGROUND: The neutrophil alkaline phosphatase (NAP) score is a valuable test for the diagnosis of myeloproliferative neoplasms, but it has still manually rated. Therefore, we developed a semi-automatic rating method using Photoshop® and Image-J, called NAP-PS-IJ. METHODS: Neutrophil alkaline phosphatase staining was conducted with Tomonaga's method to films of peripheral blood taken from three healthy volunteers. At least 30 neutrophils with NAP scores from 0 to 5+ were observed and taken their images. From which the outer part of neutrophil was removed away with Image-J. These were binarized with two different procedures (P1 and P2) using Photoshop® . NAP-positive area (NAP-PA) and granule (NAP-PGC) were measured and counted with Image-J. RESULTS: The NAP-PA in images binarized with P1 significantly (P < 0.05) differed between images with NAP scores from 0 to 3+ (group 1) and those from 4+ to 5+ (group 2). The original images in group 1 were binarized with P2. NAP-PGC of them significantly (P < 0.05) differed among all four NAP score groups. The mean NAP-PGC with NAP-PS-IJ indicated a good correlation (r = 0.92, P < 0.001) to results by human examiners. CONCLUSIONS: The sensitivity and specificity of NAP-PS-IJ were 60% and 92%, which might be considered as a prototypic method for the full-automatic rating NAP score.
Authors: A Shibata; J M Bennett; G L Castoldi; D Catovsky; G Flandrin; E S Jaffe; I Katayama; K Nanba; F Schmalzl; L T Yam Journal: Clin Lab Haematol Date: 1985