Literature DB >> 2737463

Detection of enterobacterial common antigen on bacterial cell surfaces by colony-immunoblotting: effect of its linkage to lipopolysaccharide.

U Meier-Dieter1, G Acker, H Mayer.   

Abstract

A colony-immunoblotting procedure is described which allows a quick screening of high numbers of bacteria for their Enterobacterial Common Antigen phenotype. In this assay the intensity of reaction is dependent on the carrier to which ECA is linked and which anchors ECA in the outer membrane. Bacteria containing LPS-linked ECA react stronger in this and in other immunoassays than bacteria containing only the phospholipid-linked ECA.

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Year:  1989        PMID： 2737463     DOI： 10.1016/0378-1097(89)90489-8

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  6 in total

1.  O acetylation of the enterobacterial common antigen polysaccharide is catalyzed by the product of the yiaH gene of Escherichia coli K-12.

Authors:  Junko Kajimura; Arifur Rahman; James Hsu; Matthew R Evans; Kevin H Gardner; Paul D Rick
Journal:  J Bacteriol       Date:  2006-08-25       Impact factor: 3.490

2.  Truncated enterohemorrhagic Escherichia coli (EHEC) O157:H7 intimin (EaeA) fusion proteins promote adherence of EHEC strains to HEp-2 cells.

Authors:  M L McKee; A D O'Brien
Journal:  Infect Immun       Date:  1996-06       Impact factor: 3.441

3.  Identification and biosynthesis of cyclic enterobacterial common antigen in Escherichia coli.

Authors:  Paul J A Erbel; Kathleen Barr; Ninguo Gao; Gerrit J Gerwig; Paul D Rick; Kevin H Gardner
Journal:  J Bacteriol       Date:  2003-03       Impact factor: 3.490

4.  Investigation of enterohemorrhagic Escherichia coli O157:H7 adherence characteristics and invasion potential reveals a new attachment pattern shared by intestinal E. coli.

Authors:  M L McKee; A D O'Brien
Journal:  Infect Immun       Date:  1995-05       Impact factor: 3.441

5.  ECA-immunogenicity of Proteus mirabilis strains.

Authors:  Katarzyna Anna Duda; Katarzyna Teresa Duda; Agnieszka Beczała; Katarzyna Kasperkiewicz; Joanna Radziejewska-Lebrecht; Mikael Skurnik
Journal:  Arch Immunol Ther Exp (Warsz)       Date:  2009-03-31       Impact factor: 4.291

6.  Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspA.

Authors:  Qing Liu; Xuegang Shen; Xiaoping Bian; Qingke Kong
Journal:  BMC Microbiol       Date:  2020-06-08       Impact factor: 3.605
  6 in total

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