| Literature DB >> 27374588 |
Lanxin Luo1, Yan Cui2, Shuting Zhang3, Lingxi Li2, Yuanyuan Li4, Peiyu Zhou4, Baoshan Sun5.
Abstract
To develop an efficient method for large preparation of various individual polyphenols from white grape skins (Fernão Pires; Vitis vinifera) by preparative high-speed counter-current chromatography (HSCCC) and preparative-HPLC, an optimized preparative HSCCC condition with two-phase solvent system composed of Hex-EtOAc-H2O (1:50:50, v/v) was used to separate grape skin polyphenols into various fractions. Both the tail-head and head-tail elution modes were used with a flow rate of 3.0ml/min and a rotary speed of 950rpm. Afterwards, a preparative-HPLC separation was applied to isolate individual polyphenols in each of the fractions from HSCCC. Total of 7 fractions (Fraction A to G) were obtained from grape skin extract by HSCCC. After preparative-HPLC isolation, fifteen individual compounds were obtained, most of which presented high yields and purity (all over 90%). The HSCCC method followed with preparative-HPLC appeared to be convenient and economical, constituting an efficient strategy for the isolation of grape skin polyphenols.Entities:
Keywords: (+)-Catechin (PubChem CID: 107957); (+)-Catechin-phloroglucinol derivative (PubChem CID: 14009031); (−)-Epicatechin (PubChem CID: 72276); Astilbin (PubChem CID: 119258); Grape skins; HPLC separation; High-speed counter-current chromatography; Phloroglucinol (PubChem CID: 359); Polyphenols; Procyanidin B1 (PubChem CID: 11250133); Procyanidin B1 3-gallate (PubChem CID: 72193635); Procyanidin B2 (PubChem CID: 122738); Procyanidin B3 (PubChem CID: 146798); Procyanidin B4 (PubChem CID: 147299); Procyanidin C1 (PubChem CID: 169853); trans-Coutaric acid (PubChem CID: 57517924)
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Year: 2016 PMID： 27374588 DOI： 10.1016/j.foodchem.2016.06.009
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514