| Literature DB >> 27373165 |
Aharon G Freud1, Karen A Keller2, Steven D Scoville3, Bethany L Mundy-Bosse4, Stephanie Cheng2, Youssef Youssef2, Tiffany Hughes4, Xiaoli Zhang5, Xiaokui Mo5, Pierluigi Porcu4, Robert A Baiocchi4, Jianhua Yu4, William E Carson6, Michael A Caligiuri4.
Abstract
Human natural killer (NK) cells develop in secondary lymphoid tissues (SLTs) through distinct stages. We identified two SLT lineage (Lin)(-)CD34(-)CD117(+/-)CD94(+)CD16(-) "stage 4" subsets according to expression of the C-type lectin-like surface-activating receptor, NKp80: NKp80(-) (stage "4a") and NKp80(+) (stage "4b"). Whereas stage 4b cells expressed more of the transcription factors T-BET and EOMES, produced interferon-gamma, and were cytotoxic, stage 4a cells expressed more of the transcription factors RORγt and AHR and produced interleukin-22, similar to SLT Lin(-)CD34(-)CD117(+)CD94(-)CD16(-) "stage 3" cells, whose phenotype overlaps with that of group 3 innate lymphoid cells (ILC3s). Co-culture with dendritic cells or transplantation into immunodeficient mice produced mature NK cells from stage 3 and stage 4a populations. These data identify NKp80 as a marker of NK cell maturity in SLTs and support a model of human NK cell development through a stage 4a intermediate with ILC3-associated features.Entities:
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Year: 2016 PMID： 27373165 PMCID： PMC4970225 DOI： 10.1016/j.celrep.2016.05.095
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423