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Literature DB >> 27372751

Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes.

Abstract

ATP-dependent chromatin remodeling complexes carry out diverse transformations of chromatin. Understanding their mechanisms requires assays that can monitor the kinetics or chromatin remodeling. In this chapter, we describe complimentary native gel-based and FRET-based methods for assaying the kinetics of ATP-driven nucleosome sliding. These methods can be readily adapted to investigate other types of nucleosomal transformations carried out by chromatin remodeling ATPases.

Entities: Chemical

Keywords: Chromatin remodeling; Enzyme kinetics; Fluorescence resonance energy transfer; Nucleosome labeling

Mesh：

Substances：

Year: 2016 PMID： 27372751 DOI： 10.1016/bs.mie.2016.01.015

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

11 in total

1. The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h.

Authors: Nathan Gamarra; Stephanie L Johnson; Michael J Trnka; Alma L Burlingame; Geeta J Narlikar
Journal: Elife Date: 2018-04-17 Impact factor: 8.140

2. Molecular organization of the early stages of nucleosome phase separation visualized by cryo-electron tomography.

Authors: Meng Zhang; César Díaz-Celis; Bibiana Onoa; Cristhian Cañari-Chumpitaz; Katherinne I Requejo; Jianfang Liu; Michael Vien; Eva Nogales; Gang Ren; Carlos Bustamante
Journal: Mol Cell Date: 2022-07-30 Impact factor: 19.328

3. SWI/SNF senses carbon starvation with a pH-sensitive low-complexity sequence.

Authors: J Ignacio Gutierrez; Gregory P Brittingham; Yonca Karadeniz; Kathleen D Tran; Arnob Dutta; Alex S Holehouse; Craig L Peterson; Liam J Holt
Journal: Elife Date: 2022-02-07 Impact factor: 8.713

4. Reconstitution of the SARS-CoV-2 ribonucleosome provides insights into genomic RNA packaging and regulation by phosphorylation.

Authors: Christopher R Carlson; Armin N Adly; Maxine Bi; Yifan Cheng; David O Morgan
Journal: bioRxiv Date: 2022-05-24

5. The Yeast INO80 Complex Operates as a Tunable DNA Length-Sensitive Switch to Regulate Nucleosome Sliding.

Authors: Coral Y Zhou; Stephanie L Johnson; Laura J Lee; Adam D Longhurst; Sean L Beckwith; Matthew J Johnson; Ashby J Morrison; Geeta J Narlikar
Journal: Mol Cell Date: 2018-02-15 Impact factor: 17.970

6. Transient Kinetic Analysis of SWR1C-Catalyzed H2A.Z Deposition Unravels the Impact of Nucleosome Dynamics and the Asymmetry of Histone Exchange.

Authors: Raushan K Singh; Jiayl Fan; Nathan Gioacchini; Shinya Watanabe; Osman Bilsel; Craig L Peterson
Journal: Cell Rep Date: 2019-04-09 Impact factor: 9.423

Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes.

1. The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h.

2. Molecular organization of the early stages of nucleosome phase separation visualized by cryo-electron tomography.

3. SWI/SNF senses carbon starvation with a pH-sensitive low-complexity sequence.

4. Reconstitution of the SARS-CoV-2 ribonucleosome provides insights into genomic RNA packaging and regulation by phosphorylation.

5. The Yeast INO80 Complex Operates as a Tunable DNA Length-Sensitive Switch to Regulate Nucleosome Sliding.

6. Transient Kinetic Analysis of SWR1C-Catalyzed H2A.Z Deposition Unravels the Impact of Nucleosome Dynamics and the Asymmetry of Histone Exchange.

7. Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.

8. A basic motif anchoring ISWI to nucleosome acidic patch regulates nucleosome spacing.

9. Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.

10. Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage.