| Literature DB >> 27371824 |
Huayu Xiong1, Huiling Zheng1, Wei Wang1, Jichao Liang1, Wei Wen1, Xiuhua Zhang1, Shengfu Wang2.
Abstract
Herein, we report a convenient approach to purify water-soluble dihydrolipoic acid (DHLA)-capped Ag nanoclusters (Ag NCs) by pH-induced precipitation under acidic conditions. The fluorescence of Ag NCs could be completely recovered by re-dispersing the precipitate into a basic solution using DHLA and NaBH4 as stabilizing ligands and etching reagent. DHLA-Ag NCs-doped agarose hydrogels have been prepared to monitor pH with a wide range from 8.0 to 4.0. When pH decreased, the fluorescence of the hydrogels under a UV lamp decreased and completely disappeared after pH 5. The DHLA-Ag NCs-doped agarose hydrogels biosensor showed low cytotoxicity and long stability. Accordingly, a fluorescent pH sensor for bacterial monitoring has been employed based on the "OFF-ON" signal switch of the Ag NCs-agarose hydrogel.Entities:
Keywords: Ag nanoclusters; Bacterial monitoring; Fluorescence sensor; Fluorescent pH sensor; Signal off-on
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Year: 2016 PMID： 27371824 DOI： 10.1016/j.bios.2016.06.038
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618