Nuria Boronat-Echeverría1, Héctor Aguirre-Mariscal2, Margarita Carmolinga-Ponce3, Yolanda Sevilla-Delgado4, Ricardo Miceli-Flores5, Aisha Kennedy-Padilla6, Juan Manuel Mejía-Aranguré7. 1. Department of Pediatric Otorhinolaryngology, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av Cuauhtemoc 330, México City 06720, Mexico. Electronic address: boenorl@live.com.mx. 2. Department of Pediatric Otorhinolaryngology, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av Cuauhtemoc 330, México City 06720, Mexico. Electronic address: hectororl@hotmail.com. 3. Medical Research Unit Infectious and Parasitic Diseases, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Av Cuauhtemoc 330, México City 06720, Mexico. Electronic address: margaritacamorlinga@yahoo.com. 4. Department of Pediatric Otorhinolaryngology, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av Cuauhtemoc 330, México City 06720, Mexico. Electronic address: yolanda.sevilla@imss.gob.mx. 5. Department of Pediatric Otorhinolaryngology, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av Cuauhtemoc 330, México City 06720, Mexico. Electronic address: drmicelli@hotmail.com. 6. Department of Pediatric Otorhinolaryngology, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av Cuauhtemoc 330, México City 06720, Mexico. Electronic address: aishakp@yahoo.com. 7. Unit of Research in Clinical Epidemiology, High Specialty Medical Care Unit of the Pediatric Hospital of the Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av Cuauhtemoc 330, México City 06720, Mexico. Electronic address: juan.mejiaa@imss.gob.mx.
Abstract
OBJECTIVES: The study aimed to demonstrate Helicobacter pylori presence in otitis media with effusion (OME) and its association with symptomatology of gastroesophageal reflux disease (GERD). METHODS: In a cohort study, 69 effusions were collected during tympanostomy tube insertion for H. pylori detection using PCR and ELISA. Validated questionnaires were performed according to age for clinical diagnosis of GERD; chi-square ×2 statistical analysis was made. RESULTS: Eight of the 69 ear effusions (5.7%) were positive for H. pylori detection using ELISA. Two patients (2.9%) had positive results for H. pylori detection using ELISA and PCR. These eight patients had positive results too in GERD questionnaires. None of the patients with negative/suspect questionnaires had positive results for H. pylori. We found statistical association between the results of ELISA, PCR and questionnaires (×2, p = 0.001). CONCLUSIONS: The H. pylori presence in effusions varies widely, in our population the frequency was lower than other reports. We found strong association between H. pylori in effusions and positive GERD questionnaires. The bacterium role in OME chronicity is not clear, but this study supports the GERD participation in OME pathogenesis.
OBJECTIVES: The study aimed to demonstrate Helicobacter pylori presence in otitis media with effusion (OME) and its association with symptomatology of gastroesophageal reflux disease (GERD). METHODS: In a cohort study, 69 effusions were collected during tympanostomy tube insertion for H. pylori detection using PCR and ELISA. Validated questionnaires were performed according to age for clinical diagnosis of GERD; chi-square ×2 statistical analysis was made. RESULTS: Eight of the 69 ear effusions (5.7%) were positive for H. pylori detection using ELISA. Two patients (2.9%) had positive results for H. pylori detection using ELISA and PCR. These eight patients had positive results too in GERD questionnaires. None of the patients with negative/suspect questionnaires had positive results for H. pylori. We found statistical association between the results of ELISA, PCR and questionnaires (×2, p = 0.001). CONCLUSIONS: The H. pylori presence in effusions varies widely, in our population the frequency was lower than other reports. We found strong association between H. pylori in effusions and positive GERD questionnaires. The bacterium role in OME chronicity is not clear, but this study supports the GERD participation in OME pathogenesis.
Authors: Stefan A Boers; Marjolein de Zeeuw; Ruud Jansen; Marc P van der Schroeff; Annemarie M C van Rossum; John P Hays; Suzanne J C Verhaegh Journal: Eur J Clin Microbiol Infect Dis Date: 2018-02-05 Impact factor: 3.267
Authors: Laith Khasawneh; Adi H Khassawneh; Khalid A Kheirallah; Giampiero Neri; Giulio Filograna Pignatelli; Hasan Ibrahim Al-Balas; Stefano Martinotti; Abdel-Hameed Al-Mistarehi Journal: Ann Med Surg (Lond) Date: 2021-01-24