Xinyi Su1, Queenie S W Tan2, Bhav H Parikh2, Alison Tan2, Milan N Mehta2, Yeo Sia Wey3, Sai Bo Bo Tun3, Ling-Jun Li3, Xiao-Yan Han4, Tien Y Wong5, Walter Hunziker6, Chi D Luu7, Yuji Owada8, Veluchamy A Barathi5, Samuel S Zhang9, Shyam S Chaurasia10. 1. Singapore Eye Research Institute Singapore National Eye Centre, Singapore 2Institute of Molecular Cell Biology, SIPRAD (IMCB-SERI Program in Retinal Angiogenic Diseases), A*STAR, Singapore 3Department of Ophthalmology, Yong Loo Lin School of Medicine, Nat. 2. Singapore Eye Research Institute Singapore National Eye Centre, Singapore 2Institute of Molecular Cell Biology, SIPRAD (IMCB-SERI Program in Retinal Angiogenic Diseases), A*STAR, Singapore. 3. Singapore Eye Research Institute Singapore National Eye Centre, Singapore. 4. Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. 5. Singapore Eye Research Institute Singapore National Eye Centre, Singapore 3Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 4Ophthalmology and Visual Sciences Academic Clinical Program, DUKE-NUS Gra. 6. Institute of Molecular Cell Biology, SIPRAD (IMCB-SERI Program in Retinal Angiogenic Diseases), A*STAR, Singapore 5Department of Physiology, National University of Singapore, Singapore. 7. Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia 7Department of Surgery (Ophthalmology), University of Melbourne, Melbourne, Australia. 8. Yamaguchi University, Department of Organ Anatomy, Ube, Japan. 9. Department of Neural and Behavioral Sciences, Penn State College of Medicine, Penn State University, Hershey, Pennsylvania, United States. 10. Ocular Immunology and Angiogenesis Lab, Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, Missouri, United States.
Abstract
PURPOSE: To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-). METHODS: Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7-/- mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot. RESULTS: Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7-/- mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7-/- mice showed significant increased areas of fluorescein leakage in the retina. FABP7-/- mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7-/- mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7-/- mice retina also have increased GFAP and VEGF expression. CONCLUSIONS: Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature.
PURPOSE: To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-). METHODS: Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7-/- mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot. RESULTS:Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7-/- mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7-/- mice showed significant increased areas of fluorescein leakage in the retina. FABP7-/- mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7-/- mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7-/- mice retina also have increased GFAP and VEGF expression. CONCLUSIONS:Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature.
Authors: Elizabeth J Crofton; Miroslav N Nenov; Yafang Zhang; Cynthia M Tapia; Joseph Donnelly; Shyny Koshy; Fernanda Laezza; Thomas A Green Journal: Neuropharmacology Date: 2020-11-09 Impact factor: 5.250
Authors: Shyam S Chaurasia; Rayne R Lim; Bhav H Parikh; Yeo Sia Wey; Bo Bo Tun; Tien Yin Wong; Chi D Luu; Rupesh Agrawal; Arkasubhra Ghosh; Alessandra Mortellaro; Elizabeth Rackoczy; Rajiv R Mohan; Veluchamy A Barathi Journal: Sci Rep Date: 2018-02-12 Impact factor: 4.379
Authors: Manuel Torres; Sebastià Parets; Javier Fernández-Díaz; Roberto Beteta-Göbel; Raquel Rodríguez-Lorca; Ramón Román; Victoria Lladó; Catalina A Rosselló; Paula Fernández-García; Pablo V Escribá Journal: Membranes (Basel) Date: 2021-11-24