Literature DB >> 27366888

Detection of Cell Proliferation Markers by Immunofluorescence Staining and Microscopy Imaging in Paraffin-Embedded Tissue Sections.

Seda Eminaga1, Polakit Teekakirikul2, Christine E Seidman3, Jonathan G Seidman3.   

Abstract

This unit describes a step-by-step protocol to detect and quantify proliferating cells in paraffin-embedded tissue sections. Two well-established markers of proliferation (incorporation of BrdU into newly synthesized DNA and expression of the nuclear protein Ki67) are detected after antigen-retrieval and subsequent immunofluorescence staining and confocal microscopy. © 2016 by John Wiley & Sons, Inc.
Copyright © 2016 John Wiley & Sons, Inc.

Entities:  

Keywords:  BrdU; Ki67; cell proliferation; immunofluorescence; immunohistochemistry

Mesh：

Substances：

Year:  2016        PMID： 27366888      PMCID： PMC5349766          DOI： 10.1002/cpmb.13

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  21 in total

Review 1.  The Ki-67 protein: from the known and the unknown.

Authors:  T Scholzen; J Gerdes
Journal:  J Cell Physiol       Date:  2000-03       Impact factor: 6.384

2.  Bromodeoxyuridine (BrdU) immunocytochemistry by exonuclease III (Exo III) digestion.

Authors:  W N Dinjens; J ten Kate; M H Lenders; E P van der Linden; F T Bosman
Journal:  Histochemistry       Date:  1992-10

3.  CELLULAR PROLIFERATION IN THE MOUSE AS REVEALED BY AUTORADIOGRAPHY WITH TRITIATED THYMIDINE.

Authors:  W L Hughes; V P Bond; G Brecher; E P Cronkite; R B Painter; H Quastler; F G Sherman
Journal:  Proc Natl Acad Sci U S A       Date:  1958-05       Impact factor: 11.205

Review 4.  Antigen retrieval immunohistochemistry: review and future prospects in research and diagnosis over two decades.

Authors:  Shan-Rong Shi; Yan Shi; Clive R Taylor
Journal:  J Histochem Cytochem       Date:  2011-01       Impact factor: 2.479

Review 5.  Proper application of antibodies for immunohistochemical detection: antibody crimes and how to prevent them.

Authors:  Richard Ivell; Katja Teerds; Gloria E Hoffman
Journal:  Endocrinology       Date:  2014-01-15       Impact factor: 4.736

6.  Assessment of proliferative activity by anti-PCNA monoclonal antibodies in formalin-fixed, paraffin-embedded samples and correlation with mitotic index.

Authors:  G Sarli; C Benazzi; R Preziosi; P S Marcato
Journal:  Vet Pathol       Date:  1995-01       Impact factor: 2.221

7.  The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis.

Authors:  N Kee; S Sivalingam; R Boonstra; J M Wojtowicz
Journal:  J Neurosci Methods       Date:  2002-03-30       Impact factor: 2.390

8.  The functional relationship between in vivo bromodeoxyuridine labeling index and Ki-67 proliferation index in human breast cancer.

Authors:  W H Goodson; D H Moore; B M Ljung; K Chew; C Florendo; B Mayall; H S Smith; F M Waldman
Journal:  Breast Cancer Res Treat       Date:  1998-05       Impact factor: 4.872

9.  Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA.

Authors:  Levan Muskhelishvili; John R Latendresse; Ralph L Kodell; Eric B Henderson
Journal:  J Histochem Cytochem       Date:  2003-12       Impact factor: 2.479

10.  Interleukin-15 regulates proliferation and self-renewal of adult neural stem cells.

Authors:  Diego Gómez-Nicola; Beatriz Valle-Argos; Noemí Pallas-Bazarra; Manuel Nieto-Sampedro
Journal:  Mol Biol Cell       Date:  2011-04-20       Impact factor: 4.138
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1.  In focus in HCB.

Authors:  Douglas J Taatjes; Jürgen Roth
Journal:  Histochem Cell Biol       Date:  2017-09-21       Impact factor: 4.304

2.  GDF10 inhibits proliferation and epithelial-mesenchymal transition in triple-negative breast cancer via upregulation of Smad7.

Authors:  Tian Zhou; Lei Yu; Jianjun Huang; Xueke Zhao; Yanwen Li; Yaxin Hu; Yu Lei
Journal:  Aging (Albany NY)       Date:  2019-05-31       Impact factor: 5.682

3.  SBT (Composed of Panax ginseng and Aconitum carmichaeli) and Stigmasterol Enhances Nitric Oxide Production and Exerts Curative Properties as a Potential Anti-Oxidant and Immunity-Enhancing Agent.

Authors:  Na-Ra Han; Kyeoung-Cheol Kim; Ju-Sung Kim; Hi-Joon Park; Seong-Gyu Ko; Phil-Dong Moon
Journal:  Antioxidants (Basel)       Date:  2022-01-20

4.  Comparative Studies on a Standardized Subfraction of Red Onion Peel Ethanolic Extract (Plant Substance), Quercetin (Pure Compound), and Their Cell Mechanism and Metabolism on MDA-MB-231.

Authors:  Kar Xin Leong; Sin Pei Chao; Poh Chiew Siah; Shern Kwok Lim; Boon Yin Khoo
Journal:  Evid Based Complement Alternat Med       Date:  2022-09-07       Impact factor: 2.650
  4 in total

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