Saman Nassiri1, Gholamreza Houshmand2, Mostafa Feghhi1, Alireza Kheirollah3, Mohammad Bahadoram4, Nariman Nassiri5. 1. Department of Ophthalmology, Infectious Ophthalmic Research Center, Schoolof Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Khuzestan, Iran. 2. Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapour University of Medical Sciences, Ahvaz 61357-15794, Khuzestan, Iran. 3. Department of Biochemistry, Cellular&Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Khuzestan, Iran. 4. Medical Student Research Committee and Social Determinant of Health Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61357-15794, Khuzestan, Iran. 5. Jules Stein Eye Institute, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles 90095, California, USA.
Abstract
AIM: To investigate the effects of periocular injection of propranolol and celecoxib on ocular levels of vascular endothelial growth factor (VEGF) in a diabetic mouse model. METHODS: Forty 4-6wk BALB-C male mice weighing 20-25 g were used. The study groups included: non-diabetic control (group 1), diabetic control (group 2), diabetic propranolol (group 3), and diabetic celecoxib (group 4). After induction of type 1 diabetes by streptozotocin, propranolol (10 µg) and celecoxib (200 µg dissolved in carboxymethylcellulose 0.5%) were injected periocularly. The ocular level of VEGF was measured in all the study groups using enzyme-linked immuno sorbent assay (ELISA) method. RESULTS: Ocular VEGF level was significantly increased (1.25 fold) in the diabetic control group when compared to the non-diabetic group one week after induction with streptozotocin (P=0.002). Both periocular propranolol and celecoxib significantly reduced ocular VEGF levels (P=0.047 and P<0.001, respectively). The effect was more pronounced with celecoxib. CONCLUSION: The periocular administration of propranolol and celecoxib can significantly reduce ocular VEGF levels in a diabetic mouse model.
AIM: To investigate the effects of periocular injection of propranolol and celecoxib on ocular levels of vascular endothelial growth factor (VEGF) in a diabeticmouse model. METHODS: Forty 4-6wk BALB-C male mice weighing 20-25 g were used. The study groups included: non-diabetic control (group 1), diabetic control (group 2), diabeticpropranolol (group 3), and diabeticcelecoxib (group 4). After induction of type 1 diabetes by streptozotocin, propranolol (10 µg) and celecoxib (200 µg dissolved in carboxymethylcellulose 0.5%) were injected periocularly. The ocular level of VEGF was measured in all the study groups using enzyme-linked immuno sorbent assay (ELISA) method. RESULTS: Ocular VEGF level was significantly increased (1.25 fold) in the diabetic control group when compared to the non-diabetic group one week after induction with streptozotocin (P=0.002). Both periocular propranolol and celecoxib significantly reduced ocular VEGF levels (P=0.047 and P<0.001, respectively). The effect was more pronounced with celecoxib. CONCLUSION: The periocular administration of propranolol and celecoxib can significantly reduce ocular VEGF levels in a diabeticmouse model.