Thomas Müller1, Thomas Herrling2, Sven Lütge3, Lutz Lohse3, Gabi Öhm3, Katinka Jung2. 1. Department of Neurology, St. Joseph Hospital, Gartenstrasse 1, 13088 Berlin, Germany. 2. Gematria Test Lab GmbH, Berlin, Germany. 3. Department of Neurology, St. Joseph Hospital Berlin-Weissensee, Berlin, Germany.
Abstract
INTRODUCTION: Cerebrospinal fluid analysis may provide insight into the interplay between chronic inflammation and response to treatment. OBJECTIVES: To demonstrate the impact of one intrathecal triamcinolone injection on the redox potential and on ascorbyl radical appearance in the cerebrospinal fluid of chronic progressive multiple sclerosis patients. METHODS: A total of 16 patients received 40 mg triamcinolone. Electron-spin resonance spectroscopy measured the oxidation range after copper ion [Cu (II)] addition and ascorbyl-radical bioavailability. RESULTS: There was an increase of Cu (II) ion absorption, which reflects an augmented content of reduced proteins. Ascorbyl radicals were present in contrast to healthy controls according to the literature. CONCLUSION: Intrathecal steroid application alters the redox potential in cerebrospinal fluid. Our findings support the beneficial role of steroids on oxidative stress generally demonstrated by ascorbyl radical appearance. Reactive oxygen species decline is necessary for an upregulated production of reduced proteins.
INTRODUCTION: Cerebrospinal fluid analysis may provide insight into the interplay between chronic inflammation and response to treatment. OBJECTIVES: To demonstrate the impact of one intrathecal triamcinolone injection on the redox potential and on ascorbyl radical appearance in the cerebrospinal fluid of chronic progressive multiple sclerosispatients. METHODS: A total of 16 patients received 40 mg triamcinolone. Electron-spin resonance spectroscopy measured the oxidation range after copper ion [Cu (II)] addition and ascorbyl-radical bioavailability. RESULTS: There was an increase of Cu (II) ion absorption, which reflects an augmented content of reduced proteins. Ascorbyl radicals were present in contrast to healthy controls according to the literature. CONCLUSION: Intrathecal steroid application alters the redox potential in cerebrospinal fluid. Our findings support the beneficial role of steroids on oxidative stress generally demonstrated by ascorbyl radical appearance. Reactive oxygen species decline is necessary for an upregulated production of reduced proteins.
Authors: Ivan Spasojević; Zorica Stević; Aleksandra Nikolić-Kokić; David R Jones; Dusko Blagojević; Mihajlo B Spasić Journal: Redox Rep Date: 2010 Impact factor: 4.412
Authors: N Kotani; T Kushikata; H Hashimoto; F Kimura; M Muraoka; M Yodono; M Asai; A Matsuki Journal: N Engl J Med Date: 2000-11-23 Impact factor: 91.245
Authors: Thomas Müller; Stefan Barghorn; Sven Lütge; Thomas Haas; Reinhold Mueller; Bjoern Gerlach; Gabi Öhm; Katrin Eilert; Isabel Trommer; Bernhard K Mueller Journal: J Neural Transm (Vienna) Date: 2014-09-11 Impact factor: 3.575