Z He1, Y Ma1, L Li1, J Liu2, H Yang3, C Chen4, N Lin1, Y Bai1, R Ma1, R Li4, Z Wu1, J Qiao4. 1. Department of Reproduction and Genetics, Reproductive Medical Centre, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, People's Republic of China. 2. Institute of Molecular and Clinical Medicine, Kunming Medical University, Chengong New District, Kunming, People's Republic of China. 3. Department of Pathology, The First People's Hospital of Yunnan Province, Kunming, People's Republic of China. 4. Department of Obstetrics and Gynecology, Reproductive Medical Centre, Peking University Third Hospital, Haidian District, Beijing, People's Republic of China.
Abstract
Background: To explore whether endometrial receptivity is determined by osteopontin (OPN) and integrin αvβ3 expression in women with elevated serum progesterone (P) and/or oestradiol (E2) who are undergoing in vitro fertilisation (IVF). Methods: According to serum hormone levels on the day of HCG administration, 33 infertile women were divided into 3 groups: the high E2, high P, and high E2 and P groups. The control group included 11 fertile, healthy women. Endometrial biopsy was performed on ovulation day + 7 to + 8 for all study participants, and the mRNA and protein expression levels of OPN and integrin αvβ3 were analyzed. Result: No statistically significant differences regarding OPN and integrin αvβ3 expression were found between infertile patients in the high P, high E2, high E2 and P and control groups. There was no significant correlation between OPN and integrin αvβ3 staining intensity during the implantation window biopsy in any of the groups studied. Conclusion: Endometrial OPN and integrant αvβ3 expression/co-expression is not impaired during the window of implantation in patients with high P, high E2, or high E2 and P levels. The clinical value of assessing endometrial receptivity with OPN and integrin αvβ3 seems to be uncertain.
Background: To explore whether endometrial receptivity is determined by osteopontin (OPN) and integrin αvβ3 expression in women with elevated serum progesterone (P) and/or oestradiol (E2) who are undergoing in vitro fertilisation (IVF). Methods: According to serum hormone levels on the day of HCG administration, 33 infertilewomen were divided into 3 groups: the high E2, high P, and high E2 and P groups. The control group included 11 fertile, healthy women. Endometrial biopsy was performed on ovulation day + 7 to + 8 for all study participants, and the mRNA and protein expression levels of OPN and integrin αvβ3 were analyzed. Result: No statistically significant differences regarding OPN and integrin αvβ3 expression were found between infertilepatients in the high P, high E2, high E2 and P and control groups. There was no significant correlation between OPN and integrin αvβ3 staining intensity during the implantation window biopsy in any of the groups studied. Conclusion: Endometrial OPN and integrant αvβ3 expression/co-expression is not impaired during the window of implantation in patients with high P, high E2, or high E2 and P levels. The clinical value of assessing endometrial receptivity with OPN and integrin αvβ3 seems to be uncertain.
Authors: Evangelos G Papanikolaou; Efstratios M Kolibianakis; Cristina Pozzobon; Parikshit Tank; Herman Tournaye; Claire Bourgain; Andre Van Steirteghem; Paul Devroey Journal: Fertil Steril Date: 2007-06-06 Impact factor: 7.329
Authors: Jaume Ordi; Montserrat Creus; Berta Ferrer; Francisco Fábregues; Francisco Carmona; Roser Casamitjana; Juan Antonio Vanrell; Juan Balasch Journal: Int J Gynecol Pathol Date: 2002-07 Impact factor: 2.762