María José Irisarri-Gutiérrez1, Carla Muñoz-Antolí1, Lucrecia Acosta2, Lucy Anne Parker3, Rafael Toledo1, Fernando Jorge Bornay-Llinares4, José Guillermo Esteban1. 1. Área de Parasitología del Departament de Biología Cel.lular i Parasitología, Facultat de Farmàcia, Universitat de València, Valencia, Spain. 2. Área de Parasitología del Departamento de Agroquímica y Medioambiente, Facultad de Farmacia, Universidad Miguel Hernández de Elche, Alicante, Spain; Laboratorio de Análisis Clínicos, Sanatorio "Fontilles", Vall de Laguar, Alicante, Spain. 3. Departamento de Salud Pública Historia de la Ciencia y Ginecología, Universidad Miguel Hernández de Elche, Alicante, Spain. 4. Área de Parasitología del Departamento de Agroquímica y Medioambiente, Facultad de Farmacia, Universidad Miguel Hernández de Elche, Alicante, Spain.
Abstract
BACKGROUND: Hookworm eggs identification and quantification is usually carried out by Kato-Katz method. However various structures present in the smear may be confused with eggs of such parasites. OBJECTIVE: To document the presence of structures in Kato-Katz slides that could initially be misinterpreted as hookworm eggs. METHOD: 497 faecal samples were analysed by Kato-Katz technique, diphasic concentration technique, agar-plate coprocultive and larvae obtained were analysed by PCR and characterized by sequencing. RESULT: Hookworm-like eggs were found in 159 (32%) of the samples by Kato-Katz, finally identified as Caenorhabditis elegans by PCR technique. CONCLUSION: The diagnosis of human hookworm eggs, only by the use of Kato-Katz technique can lead to false positives because of similarities with eggs of other free-living worms, from wet soils like those of Rwanda that could contaminate stool samples.
BACKGROUND: Hookworm eggs identification and quantification is usually carried out by Kato-Katz method. However various structures present in the smear may be confused with eggs of such parasites. OBJECTIVE: To document the presence of structures in Kato-Katz slides that could initially be misinterpreted as hookworm eggs. METHOD: 497 faecal samples were analysed by Kato-Katz technique, diphasic concentration technique, agar-plate coprocultive and larvae obtained were analysed by PCR and characterized by sequencing. RESULT: Hookworm-like eggs were found in 159 (32%) of the samples by Kato-Katz, finally identified as Caenorhabditis elegans by PCR technique. CONCLUSION: The diagnosis of human hookworm eggs, only by the use of Kato-Katz technique can lead to false positives because of similarities with eggs of other free-living worms, from wet soils like those of Rwanda that could contaminate stool samples.
Authors: Jaco J Verweij; Eric A T Brienen; Juventus Ziem; Lawrence Yelifari; Anton M Polderman; Lisette Van Lieshout Journal: Am J Trop Med Hyg Date: 2007-10 Impact factor: 2.345
Authors: Marie-Anne Félix; Richard Jovelin; Céline Ferrari; Shery Han; Young Ran Cho; Erik C Andersen; Asher D Cutter; Christian Braendle Journal: BMC Evol Biol Date: 2013-01-12 Impact factor: 3.260