Anna Prigitano1, Maria Carmela Esposto1, Arianna Biffi2, Gabriella De Lorenzis3, Vincenza Favuzzi4, Raffaella Koncan5, Giuliana Lo Cascio6, Marisol Barao Ocampo7, Carla Colombo8, Giovanna Pizzamiglio9, Luisa Romanò1, Anna Maria Tortorano10. 1. Department of Biomedical Sciences for Health, Università degli Studi di Milano, Via Pascal 36, 20133 Milano, Italy. 2. Cystic Fibrosis Microbiology Laboratory and Cystic Fibrosis Center, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano, Via Francesco Sforza 35, 20122 Milano, Italy. 3. Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy. 4. Pathological Anatomy Unit, Department of Diagnostic and Public Health, Università di Verona, Policlinico G.B. Rossi - P.le L.A. Scuro 10, 37134 Verona, Italy. 5. Microbiology and Virology Unit, Department of Diagnostic and Public Health, Università di Verona, Policlinico G.B. Rossi - P.le L.A. Scuro 10, 37134 Verona, Italy. 6. Microbiology and Virology Operating Unit, Department of Pathology and Diagnostic, Azienda Ospedaliera Universitaria Integrata, Policlinico G.B. Rossi - P.le L.A. Scuro 10, 37134 Verona, Italy. 7. Cystic Fibrosis Center, Azienda Ospedaliera Universitaria Integrata, Policlinico G.B. Rossi - P.le L.A. Scuro 10, 37134 Verona, Italy. 8. Cystic Fibrosis Center, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano, Università degli Studi di Milano, Via Francesco Sforza 35, 20122 Milano, Italy. 9. Respiratory Disease Department, Cystic Fibrosis Center Adult Section, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano, Via Francesco Sforza 35, 20122 Milano, Italy. 10. Department of Biomedical Sciences for Health, Università degli Studi di Milano, Via Pascal 36, 20133 Milano, Italy. Electronic address: annamaria.tortorano@unimi.it.
Abstract
BACKGROUND: Aspergillus fumigatus is frequently recovered from respiratory secretions of cystic fibrosis (CF) patients. Azole resistance has been increasingly reported. OBJECTIVES: To assess the prevalence of azole resistance in A. fumigatus isolates from patients followed by two CF centers of northern Italy. METHODS: 423 isolates (220 patients) were screened for azole resistance. Resistance was confirmed with the EUCAST method and cyp51A gene sequencing. Microsatellite genotyping was performed and results were compared with those of environmental resistant isolates. RESULTS: No resistance was detected in one center, while 8.2% of the patients of the other center harbored resistant isolates. The TR34/L98H alteration in the cyp51A gene, present in seven cases, resulted associated with poor in-vitro activity of all tested azoles. CONCLUSIONS: The environmental origin of the resistance seems to be probable since azole resistance was found also in naïve patients and an identical microsatellite genotype in clinical and environmental isolates was observed.
BACKGROUND:Aspergillus fumigatus is frequently recovered from respiratory secretions of cystic fibrosis (CF) patients. Azole resistance has been increasingly reported. OBJECTIVES: To assess the prevalence of azole resistance in A. fumigatus isolates from patients followed by two CF centers of northern Italy. METHODS: 423 isolates (220 patients) were screened for azole resistance. Resistance was confirmed with the EUCAST method and cyp51A gene sequencing. Microsatellite genotyping was performed and results were compared with those of environmental resistant isolates. RESULTS: No resistance was detected in one center, while 8.2% of the patients of the other center harbored resistant isolates. The TR34/L98H alteration in the cyp51A gene, present in seven cases, resulted associated with poor in-vitro activity of all tested azoles. CONCLUSIONS: The environmental origin of the resistance seems to be probable since azole resistance was found also in naïve patients and an identical microsatellite genotype in clinical and environmental isolates was observed.
Authors: A Arastehfar; A Carvalho; J Houbraken; L Lombardi; R Garcia-Rubio; J D Jenks; O Rivero-Menendez; R Aljohani; I D Jacobsen; J Berman; N Osherov; M T Hedayati; M Ilkit; D James-Armstrong; T Gabaldón; J Meletiadis; M Kostrzewa; W Pan; C Lass-Flörl; D S Perlin; M Hoenigl Journal: Stud Mycol Date: 2021-05-10 Impact factor: 16.097
Authors: L Bernal-Martínez; H Gil; O Rivero-Menéndez; S Gago; M Cuenca-Estrella; E Mellado; A Alastruey-Izquierdo Journal: Antimicrob Agents Chemother Date: 2017-11-22 Impact factor: 5.191
Authors: Jochem B Buil; Rasmus K Hare; Bas J Zwaan; Maiken C Arendrup; Willem J G Melchers; Paul E Verweij Journal: PLoS Pathog Date: 2019-08-22 Impact factor: 6.823