OBJECTIVE: To analyze the monosaccharide composition and the molar ratio of Paeoniae Radix Alba polysaccharide(PYPS). METHODS: Pre-column derivation HPLC method was developed to determine the monosaccharide composition and the molar ratio of PYPS from Paeonia lactiflora root of different growth years and obtained by graded alcohol precipitation. RESULTS: The results of HPLC analysis showed that PYPS consisted of Man, Rha, Glc, Ara, Gal and Xyl with the molar ratio of 156.26 : 0.73 : 0.85 : 1.03 : 1.17 : 0.79. Glc molar ratio increased gradually with the increasing of growth years, while the other five monosaccharides decreased. CONCLUSION: Precolumn derivation HPLC method can be used to determine the monosaccharides in PYPS, molar ratio are differently in the different growth years and molecular weight of the PYPS, Glc is the main monosaccharide of PYPS.
OBJECTIVE: To analyze the monosaccharide composition and the molar ratio of Paeoniae Radix Alba polysaccharide(PYPS). METHODS: Pre-column derivation HPLC method was developed to determine the monosaccharide composition and the molar ratio of PYPS from Paeonia lactiflora root of different growth years and obtained by graded alcohol precipitation. RESULTS: The results of HPLC analysis showed that PYPS consisted of Man, Rha, Glc, Ara, Gal and Xyl with the molar ratio of 156.26 : 0.73 : 0.85 : 1.03 : 1.17 : 0.79. Glc molar ratio increased gradually with the increasing of growth years, while the other five monosaccharides decreased. CONCLUSION: Precolumn derivation HPLC method can be used to determine the monosaccharides in PYPS, molar ratio are differently in the different growth years and molecular weight of the PYPS, Glc is the main monosaccharide of PYPS.