Ahmad Asghari1, Ghasem Akbari2, Afshin Meghdadi3, Pejman Mortazavi4. 1. Assistant Professor, Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Design and supervised all phases of the study, manuscript writing. 2. Assistant Professor, Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Design of the study, analysis and interpretation of data. 3. Graduate student, Science and Research Branch, Islamic Azad University, Tehran, Iran. Technical procedures, acquisition and interpretation of data. 4. Associate Professor Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Histological examinations.
Abstract
PURPOSE: To investigate the protective effect of metformin on testicular ischemia/reperfusion (I/R) injury in rats. METHODS: Eighteen adult male Wistar rats were randomly divided into three experimental groups (n=6), as follows: Sham, I/R, and Metformin. 1-hour ischemia was induced by the left testicular artery and vein clipping followed by 7 days of reperfusion. Metformin (100 mg/kg) was administrated orally for 7 days via oral gavage after ischemic period. At the end of trial, the left testis was removed for histological analysis and oxidative stress measurement. RESULTS: I/R reduced superoxide dismutase (SOD) activities and testicular Johnsen's scores accompanied by an elevation in malondialdehyde (MDA) and myeloperoxidase (MPO) levels in comparison with the sham group (P < 0.05). Compared to I/R group, metformin restored testicular Johnsen's scores, SOD activity, MDA and MPO levels (P < 0.05). CONCLUSION: Metformin has a protective effect against I/R injury on the testis.
PURPOSE: To investigate the protective effect of metformin on testicular ischemia/reperfusion (I/R) injury in rats. METHODS: Eighteen adult male Wistar rats were randomly divided into three experimental groups (n=6), as follows: Sham, I/R, and Metformin. 1-hour ischemia was induced by the left testicular artery and vein clipping followed by 7 days of reperfusion. Metformin (100 mg/kg) was administrated orally for 7 days via oral gavage after ischemic period. At the end of trial, the left testis was removed for histological analysis and oxidative stress measurement. RESULTS: I/R reduced superoxide dismutase (SOD) activities and testicular Johnsen's scores accompanied by an elevation in malondialdehyde (MDA) and myeloperoxidase (MPO) levels in comparison with the sham group (P < 0.05). Compared to I/R group, metformin restored testicular Johnsen's scores, SOD activity, MDA and MPO levels (P < 0.05). CONCLUSION:Metformin has a protective effect against I/R injury on the testis.