Edip Erdal Yılmaz1, Zübeyir Bozdağ2, Ibrahim Ibiloğlu3, Zülfü Arıkanoğlu4, Ümit Can Yazgan5, Ibrahim Kaplan6, Metehan Gümüş7, Sabri Selçuk Atamanalp8. 1. MD, Department of General Surgery, Diyarbakır Gazi Yaşargil Education and Research Hospital, Turkey. Intellectual and scientific content of the study, design the protocol, surgical procedures, acquisition and interpretation of data, statistical analysis, manuscript writing. 2. Assistant Professor, Department of General Surgery, Faculty of Medicine, Dicle University, Turkey. Design the protocol, surgical procedures, acquisition and interpretation of data, statistical analysis, manuscript writing. 3. Assistant Professor, Department of Pathology, Faculty of Medicine, Dicle University, Turkey. Surgical procedures, acquisition of data. 4. Assosciate Professor, Department of General Surgery, Faculty of Medicine, Dicle University, Turkey. Surgical procedures, acquisition and interpretation of data, statistical analysis. 5. Assistant Professor, Department of Physiology, Faculty of Medicine, Zirve University, Turkey. Surgical procedures, statistical analysis. 6. Assistant Professor, Department of Biochemistry, Faculty of Medicine, Dicle University, Turkey. Surgical procedures, acquisition and interpretation of data, statistical analysis. 7. Assosciate Professor, Department of General Surgery, Faculty of Medicine, Dicle University, Turkey. Statistical analysis, acquisition and interpretation of data, manuscript writing, critical revision. 8. Professor, Head, Department of General Surgery, Faculty of Medicine, Ataturk University, Turkey. Manuscript writing, critical revision.
Abstract
PURPOSE: To investigate the therapeutic effects of ellagic acid on L-arginin ınduced acute pancreatitis in rats. METHODS: Thirty-two were split into four groups. Group 1 (control) rats were performed only laparotomy, no drugs were administered. Group 2 (control+EA) rats were administered 85mg/kg EA orally. Rats were sacrificed by cardiac puncture 24 hours after the administration. Group3 (AP) 24 hours after intraperitoneal L-arginine administration, rats were sacrificed by cardiac puncture. Group 4 (EA)-(AP): 85mg/kg EA was administered orally after the L-arginine administration. 24 hours later, rats were sacrificed by cardiac puncture. Serum TNF-α, IL-1β, IL-6, total oxidative status (TOS), total antioxidant capacity (TAC), amylase levels were determined in all groups. RESULTS: Group 3 (AP) rats showed significantly raised TOS level as compared to Group1 (control) rats (p<0.001). Following the EA therapy, a decrease in TOS was observed in Group 4 (AP+EA). TAC levels were significantly raised in the Group 4 (AP+EA) compared to the Group 3 (AP) (p=0.003). Group 3 (AP) showed significantly increased TNF-α, IL-1β and IL-6 serum levels as compared to Group 4 (AP+EA). Histopathological changes were supported our result. CONCLUSION: The healing effects of ellagic acid on inflammatory and oxidative stress were confirmed by histopathological and biochemical evaluations of the pancreatic tissue.
PURPOSE: To investigate the therapeutic effects of ellagic acid on L-arginin ınduced acute pancreatitis in rats. METHODS: Thirty-two were split into four groups. Group 1 (control) rats were performed only laparotomy, no drugs were administered. Group 2 (control+EA) rats were administered 85mg/kg EA orally. Rats were sacrificed by cardiac puncture 24 hours after the administration. Group3 (AP) 24 hours after intraperitoneal L-arginine administration, rats were sacrificed by cardiac puncture. Group 4 (EA)-(AP): 85mg/kg EA was administered orally after the L-arginine administration. 24 hours later, rats were sacrificed by cardiac puncture. Serum TNF-α, IL-1β, IL-6, total oxidative status (TOS), total antioxidant capacity (TAC), amylase levels were determined in all groups. RESULTS: Group 3 (AP) rats showed significantly raised TOS level as compared to Group1 (control) rats (p<0.001). Following the EA therapy, a decrease in TOS was observed in Group 4 (AP+EA). TAC levels were significantly raised in the Group 4 (AP+EA) compared to the Group 3 (AP) (p=0.003). Group 3 (AP) showed significantly increased TNF-α, IL-1β and IL-6 serum levels as compared to Group 4 (AP+EA). Histopathological changes were supported our result. CONCLUSION: The healing effects of ellagic acid on inflammatory and oxidative stress were confirmed by histopathological and biochemical evaluations of the pancreatic tissue.