Teruko Honda1, Hiroyuki Inagawa2. 1. Department of Medical Technology, School of Life and Environmental Science, Azabu University, Sagamihara-shi, Kanagawa, Japan hondat@azabu-u.ac.jp. 2. Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kita-gun, Kagawa, Japan Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niitsu-shi, Niigata, Japan.
Abstract
BACKGROUND/AIM: Monocytes migrate into the tissue where they differentiate into various types of macrophages with tissue-specific characteristics. When human monocytes are co-cultured with colon cancer cells they exhibit increased mRNA expression of angiogenesis- and signaling pathway-related genes; however, this increase is suppressed by pretreatment with low-dose lipopolysaccharide (LPS). Thus, LPS-treated human monocytes may be useful in suppressing tumor invasion and proliferation in colon cancer. However, it is suggested that the characteristics of tumor-associated macrophages may differ depending on the type of cancer. The function of human tumor-associated macrophages in hepatic cancer remains unclear. In this study, we investigated mRNA expression of various genes in LPS-treated human monocytes following interaction with hepatic cancer cells. MATERIALS AND METHODS: The human monocyte cell line THP-1 was treated with LPS and subsequently co-cultured with the human hepatic cancer cell line HepG2. mRNA expression of various factors were then analyzed using quantitative real-time polymerase chain reaction (PCR) and DNA microarray. RESULTS: The mRNA expressions of monocyte chemotactic protein-1, vascular endothelial growth factor-A, tumor necrosis factor-α, interleukin (IL)-1β, IL-8, nuclear factor-κB, RelB, signal transducer and activator of transcription 3, IL-10 and transforming growth factor-β in THP-1 cells following interaction with HepG2 cells, were suppressed by pretreatment with LPS. CONCLUSION: LPS-treated human monocytes may be useful in suppressing tumor invasion and proliferation of hepatic cancer, as well as colon cancer. The co-culture system of monocytes and cancer cells may be beneficial for evaluating antitumor effects in LPS-treated monocytes. Copyright
BACKGROUND/AIM: Monocytes migrate into the tissue where they differentiate into various types of macrophages with tissue-specific characteristics. When human monocytes are co-cultured with colon cancer cells they exhibit increased mRNA expression of angiogenesis- and signaling pathway-related genes; however, this increase is suppressed by pretreatment with low-dose lipopolysaccharide (LPS). Thus, LPS-treated human monocytes may be useful in suppressing tumor invasion and proliferation in colon cancer. However, it is suggested that the characteristics of tumor-associated macrophages may differ depending on the type of cancer. The function of humantumor-associated macrophages in hepatic cancer remains unclear. In this study, we investigated mRNA expression of various genes in LPS-treated human monocytes following interaction with hepatic cancer cells. MATERIALS AND METHODS: The human monocyte cell line THP-1 was treated with LPS and subsequently co-cultured with the humanhepatic cancer cell line HepG2. mRNA expression of various factors were then analyzed using quantitative real-time polymerase chain reaction (PCR) and DNA microarray. RESULTS: The mRNA expressions of monocyte chemotactic protein-1, vascular endothelial growth factor-A, tumor necrosis factor-α, interleukin (IL)-1β, IL-8, nuclear factor-κB, RelB, signal transducer and activator of transcription 3, IL-10 and transforming growth factor-β in THP-1 cells following interaction with HepG2 cells, were suppressed by pretreatment with LPS. CONCLUSION: LPS-treated human monocytes may be useful in suppressing tumor invasion and proliferation of hepatic cancer, as well as colon cancer. The co-culture system of monocytes and cancer cells may be beneficial for evaluating antitumor effects in LPS-treated monocytes. Copyright
Authors: Noelia M Rodriguez-Martin; Sergio Montserrat-de la Paz; Rocio Toscano; Elena Grao-Cruces; Alvaro Villanueva; Justo Pedroche; Francisco Millan; Maria C Millan-Linares Journal: Biomolecules Date: 2020-05-22