Arezoo Kiaei1, Habib Onsori2, Aylar Alijani1, Sasan Andalib3, Saeid Ghorbian4, Ebrahim Sakhinia5. 1. Department of Biology, Tabriz Branch, Islamic Azad University Tabriz, Iran; Department of Biology, College of Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran. 2. Department of Cell and Molecular Biology, Marand Branch, Islamic Azad University, Marand, Iran. 3. Department of Neurosurgery, Poursina Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran. 4. Young Researchers and Elite Club, Ahar Branch, Islamic Azad University, Ahar, Iran. 5. Connective Tissue Research Center, Department of Medical Genetics, Faculty of Medicine and Tabriz Genetic Analysis Centre (TGAC), Tabriz University of Medical Sciences, Daneshgah Street, 516661557, Tabriz, Iran. Electronic address: esakhinia@yahoo.co.uk.
Abstract
OBJECTIVE/ BACKGROUND: Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t(8;14)(q24;q32), which involves the c-myc gene (8q24) and the immunoglobulin heavy-chain (IgH) locus (14q32), accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma (DLBCL). Long-distance polymerase chain reaction (LD-PCR) assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t(8;14) c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL. METHODS: In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene (joining region and γ switch region) and the myc gene (Exons 2 and 3). RESULTS: As much as 1.85% of the samples were positive for the γ constant region and Exon 2 of the myc gene. CONCLUSION: LD-PCR can be used for the detection of t(8;14) c-myc/IgH gene rearrangement in patients with DLBCL. Copyright Â
OBJECTIVE/ BACKGROUND: Specific chromosomal translocations are found in humanleukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t(8;14)(q24;q32), which involves the c-myc gene (8q24) and the immunoglobulin heavy-chain (IgH) locus (14q32), accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma (DLBCL). Long-distance polymerase chain reaction (LD-PCR) assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t(8;14) c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL. METHODS: In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene (joining region and γ switch region) and the myc gene (Exons 2 and 3). RESULTS: As much as 1.85% of the samples were positive for the γ constant region and Exon 2 of the myc gene. CONCLUSION: LD-PCR can be used for the detection of t(8;14) c-myc/IgH gene rearrangement in patients with DLBCL. Copyright Â
Authors: Giulia Bicchierai; Luigi Rigacci; Vittorio Miele; Icro Meattini; Diego De Benedetto; Valeria Selvi; Simonetta Bianchi; Lorenzo Livi; Jacopo Nori Journal: Radiol Med Date: 2017-05-16 Impact factor: 3.469