Susan Azizmohammadi1, Sima Azizmohammadi1, Aghdas Safari2, Niloufar Kosari3, Maria Kaghazian4, Emad Yahaghi5, Mehri Seifoleslami6. 1. Department of Gynecology, Hajar Hospital, AJA University of Medical Sciences Tehran, Iran. 2. Department of Gynecology, Imam Reza Hospital, AJA University of Medical Sciences Tehran, Iran. 3. Department of Biology, California State University Northridge California, USA. 4. Department of Biology, Jundishapur University of Medical Sciences Ahvaz, Iran. 5. Department of Molecular Biology, Baqiyatallah University of Medical Sciences Tehran, Iran. 6. Department of Gynecology, Khanevadeh Hospital, AJA University of Medical Sciences Tehran, Iran.
Abstract
PURPOSE: The present study was aimed to evaluate the clinical significance of miR-100 and miR-203 in epithelial ovarian cancer (EOC) patients. METHODS: The expression levels of miR-100/203 in EOC tissue and adjacent non-cancerous samples were determined by real-time RT-PCR. Associations between miRNAs expressions and various clinicopathological characteristics were analyzed. Survival rate was determined with Kaplan-Meier and statistically analyzed with the log-rank method between groups. Survival data were evaluated through multivariate. Cox regression analysis. FINDINGS: Our findings showed that miR-100 was significantly down-regulated in EOC tissue specimens than in adjacent non-cancerous tissues. The expression level of miR-203 was significantly higher in EOC tissues compared to adjacent non-cancerous tissues. Decreased expression of miR-100 was strongly associated with high FIGO stage (P=0.012). The high expression of miR-203 was significantly correlated with advanced FIGO stage (p=0.006), advanced histological grade (p=0.03). Kaplan-Meier analysis and log-rank test have suggested that EOC patients with down-regulated miR-100 expression and up-regulated miR-203 expression have shorter overall survival when compared with patients with other expression groups (log-rank test P<0.001). Multivariate Cox proportional hazards model indicated that the status of miR-100 and miR-203 expression levels were independent predictor of overall survival in patients with EOC. CONCLUSION: Decreased expression and increased expression of miR-100 and miR-203 may be correlated with progression and poor prognosis of EOC.
PURPOSE: The present study was aimed to evaluate the clinical significance of miR-100 and miR-203 in epithelial ovarian cancer (EOC) patients. METHODS: The expression levels of miR-100/203 in EOC tissue and adjacent non-cancerous samples were determined by real-time RT-PCR. Associations between miRNAs expressions and various clinicopathological characteristics were analyzed. Survival rate was determined with Kaplan-Meier and statistically analyzed with the log-rank method between groups. Survival data were evaluated through multivariate. Cox regression analysis. FINDINGS: Our findings showed that miR-100 was significantly down-regulated in EOC tissue specimens than in adjacent non-cancerous tissues. The expression level of miR-203 was significantly higher in EOC tissues compared to adjacent non-cancerous tissues. Decreased expression of miR-100 was strongly associated with high FIGO stage (P=0.012). The high expression of miR-203 was significantly correlated with advanced FIGO stage (p=0.006), advanced histological grade (p=0.03). Kaplan-Meier analysis and log-rank test have suggested that EOC patients with down-regulated miR-100 expression and up-regulated miR-203 expression have shorter overall survival when compared with patients with other expression groups (log-rank test P<0.001). Multivariate Cox proportional hazards model indicated that the status of miR-100 and miR-203 expression levels were independent predictor of overall survival in patients with EOC. CONCLUSION: Decreased expression and increased expression of miR-100 and miR-203 may be correlated with progression and poor prognosis of EOC.
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